MED28_MOUSE
ID MED28_MOUSE Reviewed; 178 AA.
AC Q920D3; Q3U4G7; Q9CQF2;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 28;
DE AltName: Full=Mediator complex subunit 28;
GN Name=Med28; ORFNames=Fksg20;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Wang Y.-G., Gong L.;
RT "Cloning of FKSG20, a novel gene expressed in adrenal gland tumor
RT tissues.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Pancreas, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. May be part of a complex
CC containing NF2/merlin that participates in cellular signaling to the
CC actin cytoskeleton downstream of tyrosine kinase signaling pathways (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Forms a ternary complex
CC with NF2/merlin and GRB2. Binds to actin (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Membrane {ECO:0000250}. Note=May be also cytoplasmic and membrane-
CC associated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 28 family.
CC {ECO:0000305}.
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DR EMBL; AF318060; AAL26907.1; -; mRNA.
DR EMBL; AK003854; BAB23039.1; -; mRNA.
DR EMBL; AK005681; BAB24184.1; -; mRNA.
DR EMBL; AK007855; BAB25307.1; -; mRNA.
DR EMBL; AK154249; BAE32464.1; -; mRNA.
DR EMBL; BC024941; AAH24941.1; -; mRNA.
DR CCDS; CCDS19275.1; -.
DR RefSeq; NP_080171.1; NM_025895.4.
DR PDB; 6W1S; EM; 4.02 A; W=32-149.
DR PDBsum; 6W1S; -.
DR AlphaFoldDB; Q920D3; -.
DR SMR; Q920D3; -.
DR ComplexPortal; CPX-3264; Core mediator complex.
DR IntAct; Q920D3; 47.
DR MINT; Q920D3; -.
DR STRING; 10090.ENSMUSP00000115882; -.
DR PhosphoSitePlus; Q920D3; -.
DR EPD; Q920D3; -.
DR MaxQB; Q920D3; -.
DR PaxDb; Q920D3; -.
DR PeptideAtlas; Q920D3; -.
DR PRIDE; Q920D3; -.
DR ProteomicsDB; 295920; -.
DR Antibodypedia; 23086; 175 antibodies from 28 providers.
DR DNASU; 66999; -.
DR Ensembl; ENSMUST00000156481; ENSMUSP00000115882; ENSMUSG00000015804.
DR GeneID; 66999; -.
DR KEGG; mmu:66999; -.
DR UCSC; uc012dvz.1; mouse.
DR CTD; 80306; -.
DR MGI; MGI:1914249; Med28.
DR VEuPathDB; HostDB:ENSMUSG00000015804; -.
DR eggNOG; ENOG502QSN9; Eukaryota.
DR GeneTree; ENSGT00390000006192; -.
DR HOGENOM; CLU_129063_0_0_1; -.
DR InParanoid; Q920D3; -.
DR OMA; MQPSPQH; -.
DR OrthoDB; 1504193at2759; -.
DR PhylomeDB; Q920D3; -.
DR TreeFam; TF326988; -.
DR BioGRID-ORCS; 66999; 13 hits in 72 CRISPR screens.
DR ChiTaRS; Med28; mouse.
DR PRO; PR:Q920D3; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q920D3; protein.
DR Bgee; ENSMUSG00000015804; Expressed in primary oocyte and 265 other tissues.
DR ExpressionAtlas; Q920D3; baseline and differential.
DR Genevisible; Q920D3; MM.
DR GO; GO:0070847; C:core mediator complex; ISO:MGI.
DR GO; GO:0030864; C:cortical actin cytoskeleton; IDA:MGI.
DR GO; GO:0005856; C:cytoskeleton; IDA:MGI.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0051151; P:negative regulation of smooth muscle cell differentiation; IMP:MGI.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR GO; GO:0035019; P:somatic stem cell population maintenance; IMP:MGI.
DR InterPro; IPR021640; Mediator_Med28.
DR PANTHER; PTHR13512; PTHR13512; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Actin-binding; Activator; Coiled coil; Cytoplasm; Membrane;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..178
FT /note="Mediator of RNA polymerase II transcription subunit
FT 28"
FT /id="PRO_0000113982"
FT REGION 1..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 109..145
FT /evidence="ECO:0000255"
FT COMPBIAS 11..25
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 108
FT /note="D -> H (in Ref. 1; AAL26907)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 178 AA; 19538 MW; 947FDA98E806EA13 CRC64;
MAASLGGMFT GQPPGPPPPP PGLPGQASLL QAAPGAPRPS NSTLVDELES SFEACFASLV
SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL QLSVQKPDQV IKEDVSELRS
ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH KKPADMPQGS LAFLEQASAN IPAPLKQT
