ID   MED28_RAT               Reviewed;         178 AA.
AC   P68943;
DT   01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 28;
DE   AltName: Full=Mediator complex subunit 28;
GN   Name=Med28;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RX   PubMed=15057822; DOI=10.1038/nature02426;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP   COMPLEX WITH CCNC; CDK8; MED1; MED6; MED7; MED10; MED12; MED17; MED21;
RP   MED24; MED27; MED28; USP47; MED30 AND MED31.
RX   PubMed=12149480; DOI=10.1073/pnas.162424199;
RA   Brower C.S., Sato S., Tomomori-Sato C., Kamura T., Pause A., Stearman R.,
RA   Klausner R.D., Malik S., Lane W.S., Sorokina I., Roeder R.G., Conaway J.W.,
RA   Conaway R.C.;
RT   "Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that
RT   can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:10353-10358(2002).
CC   -!- FUNCTION: May be part of a complex containing NF2/merlin that
CC       participates in cellular signaling to the actin cytoskeleton downstream
CC       of tyrosine kinase signaling pathways (By similarity). Component of the
CC       Mediator complex, a coactivator involved in the regulated transcription
CC       of nearly all RNA polymerase II-dependent genes. Mediator functions as
CC       a bridge to convey information from gene-specific regulatory proteins
CC       to the basal RNA polymerase II transcription machinery. Mediator is
CC       recruited to promoters by direct interactions with regulatory proteins
CC       and serves as a scaffold for the assembly of a functional preinitiation
CC       complex with RNA polymerase II and the general transcription factors.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Forms a ternary complex with NF2/merlin and GRB2. Binds to
CC       actin (By similarity). Component of the Mediator complex, which is
CC       probably composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11,
CC       MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20,
CC       MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31,
CC       CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits
CC       form a distinct module termed the CDK8 module. Mediator containing the
CC       CDK8 module is less active than Mediator lacking this module in
CC       supporting transcriptional activation. Individual preparations of the
CC       Mediator complex lacking one or more distinct subunits have been
CC       variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. {ECO:0000250,
CC       ECO:0000269|PubMed:12149480}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC       Membrane {ECO:0000250}. Note=May be also cytoplasmic and membrane-
CC       associated. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 28 family.
CC       {ECO:0000305}.
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DR   EMBL; AABR03090012; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001100687.1; NM_001107217.1.
DR   AlphaFoldDB; P68943; -.
DR   SMR; P68943; -.
DR   STRING; 10116.ENSRNOP00000004912; -.
DR   PaxDb; P68943; -.
DR   Ensembl; ENSRNOT00000004912; ENSRNOP00000004912; ENSRNOG00000003592.
DR   GeneID; 305391; -.
DR   KEGG; rno:305391; -.
DR   CTD; 80306; -.
DR   RGD; 1305875; Med28.
DR   eggNOG; ENOG502QSN9; Eukaryota.
DR   GeneTree; ENSGT00390000006192; -.
DR   HOGENOM; CLU_129063_0_0_1; -.
DR   InParanoid; P68943; -.
DR   OMA; MQPSPQH; -.
DR   OrthoDB; 1504193at2759; -.
DR   PhylomeDB; P68943; -.
DR   TreeFam; TF326988; -.
DR   PRO; PR:P68943; -.
DR   Proteomes; UP000002494; Chromosome 14.
DR   Bgee; ENSRNOG00000003592; Expressed in testis and 20 other tissues.
DR   Genevisible; P68943; RN.
DR   GO; GO:0070847; C:core mediator complex; ISO:RGD.
DR   GO; GO:0030864; C:cortical actin cytoskeleton; ISO:RGD.
DR   GO; GO:0005856; C:cytoskeleton; ISO:RGD.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0051151; P:negative regulation of smooth muscle cell differentiation; ISO:RGD.
DR   GO; GO:0035019; P:somatic stem cell population maintenance; IEA:Ensembl.
DR   GO; GO:0019827; P:stem cell population maintenance; ISO:RGD.
DR   InterPro; IPR021640; Mediator_Med28.
DR   PANTHER; PTHR13512; PTHR13512; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; Activator; Coiled coil; Cytoplasm; Membrane; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..178
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   28"
FT                   /id="PRO_0000113983"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          109..145
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        11..25
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   178 AA;  19524 MW;  73057B935817FB0B CRC64;
     MAASLGGMFA GQPPGPPPPP PGLPGQASLL QAAPGAPRPS NSTLVDELES SFEACFASLV
     SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL QLSVQKPDQV IKEDVSELRS
     ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH KKPADMPQGS LAYLEQASAN IPAPLKQT