MED29_DROME
ID MED29_DROME Reviewed; 188 AA.
AC Q7KBL8; Q0IGZ7;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 29;
DE AltName: Full=Mediator complex subunit 29;
DE AltName: Full=Protein intersex;
GN Name=ix; Synonyms=MED29; ORFNames=CG13201;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SELF-ASSOCIATION, AND
RP INTERACTION WITH DSX.
RX PubMed=12361959; DOI=10.1242/dev.129.20.4661;
RA Garrett-Engele C.M., Siegal M.L., Manoli D.S., Williams B.C., Li H.,
RA Baker B.S.;
RT "Intersex, a gene required for female sexual development in Drosophila, is
RT expressed in both sexes and functions together with doublesex to regulate
RT terminal differentiation.";
RL Development 129:4661-4675(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC female somatic sexual development. {ECO:0000250,
CC ECO:0000269|PubMed:12361959}.
CC -!- SUBUNIT: Component of the Mediator complex (By similarity). Self-
CC associates. Interacts with dsx. {ECO:0000250,
CC ECO:0000269|PubMed:12361959}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 29 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABI34154.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABI34154.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF491289; AAN37397.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF58653.1; -; Genomic_DNA.
DR EMBL; BT028773; ABI34154.1; ALT_SEQ; mRNA.
DR RefSeq; NP_610677.1; NM_136833.2.
DR AlphaFoldDB; Q7KBL8; -.
DR SMR; Q7KBL8; -.
DR BioGRID; 69950; 35.
DR IntAct; Q7KBL8; 4.
DR STRING; 7227.FBpp0087251; -.
DR PaxDb; Q7KBL8; -.
DR DNASU; 45881; -.
DR EnsemblMetazoa; FBtr0088154; FBpp0087251; FBgn0001276.
DR GeneID; 45881; -.
DR KEGG; dme:Dmel_CG13201; -.
DR CTD; 45881; -.
DR FlyBase; FBgn0001276; ix.
DR VEuPathDB; VectorBase:FBgn0001276; -.
DR eggNOG; ENOG502QRNJ; Eukaryota.
DR HOGENOM; CLU_101133_0_0_1; -.
DR InParanoid; Q7KBL8; -.
DR OMA; MPDNAGP; -.
DR OrthoDB; 1480686at2759; -.
DR PhylomeDB; Q7KBL8; -.
DR BioGRID-ORCS; 45881; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 45881; -.
DR PRO; PR:Q7KBL8; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0001276; Expressed in saliva-secreting gland and 25 other tissues.
DR ExpressionAtlas; Q7KBL8; baseline and differential.
DR Genevisible; Q7KBL8; DM.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IDA:UniProtKB.
DR GO; GO:0008134; F:transcription factor binding; IPI:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0019101; P:female somatic sex determination; TAS:FlyBase.
DR GO; GO:0035263; P:genital disc sexually dimorphic development; IMP:FlyBase.
DR GO; GO:0048804; P:imaginal disc-derived female genitalia morphogenesis; IMP:FlyBase.
DR GO; GO:0060548; P:negative regulation of cell death; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0007530; P:sex determination; IMP:FlyBase.
DR GO; GO:0007548; P:sex differentiation; TAS:FlyBase.
DR GO; GO:0018993; P:somatic sex determination; NAS:FlyBase.
DR InterPro; IPR021018; Mediator_Med29_met.
DR PANTHER; PTHR28314; PTHR28314; 1.
DR Pfam; PF11568; Med29; 1.
PE 1: Evidence at protein level;
KW Activator; Differentiation; Nucleus; Reference proteome;
KW Sexual differentiation; Transcription; Transcription regulation.
FT CHAIN 1..188
FT /note="Mediator of RNA polymerase II transcription subunit
FT 29"
FT /id="PRO_0000305703"
FT REGION 1..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 188 AA; 21147 MW; A4CCF7BFB6AAD994 CRC64;
MNPNMNMMPM SGPQMMQVMQ SSPSGPPGPV QHQQQQPPQP LQQQQQAEKL DNISRVKSLL
GPLRESMFLT IRSSAFALQQ NNLADNLKRD TGAHHVPRFD KHLEDFYACC DQIEIHLKTA
MQCLQQQNSS NHYLPGPVTP MRMETFMPDN AGPISYPTYL NTVRVHIQSA KDIHDTLISA
AQNISQAD
