ID   MED2_ASHGO              Reviewed;         343 AA.
AC   Q757M0;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 2;
DE   AltName: Full=Mediator complex subunit 2;
GN   Name=MED2; OrderedLocusNames=AEL009C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 2 family.
CC       {ECO:0000305}.
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DR   EMBL; AE016818; AAS52676.1; -; Genomic_DNA.
DR   RefSeq; NP_984852.1; NM_210206.1.
DR   AlphaFoldDB; Q757M0; -.
DR   STRING; 33169.AAS52676; -.
DR   EnsemblFungi; AAS52676; AAS52676; AGOS_AEL009C.
DR   GeneID; 4621051; -.
DR   KEGG; ago:AGOS_AEL009C; -.
DR   eggNOG; ENOG502RZB6; Eukaryota.
DR   HOGENOM; CLU_046031_0_0_1; -.
DR   InParanoid; Q757M0; -.
DR   OMA; AEMMMQQ; -.
DR   Proteomes; UP000000591; Chromosome V.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   InterPro; IPR021017; Mediator_Med2_fun.
DR   Pfam; PF11214; Med2; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..343
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   2"
FT                   /id="PRO_0000302013"
FT   REGION          105..141
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          252..277
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        105..131
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   343 AA;  37087 MW;  4B4DA2DAF9B78B18 CRC64;
     MIKELQGGNM ATKFKTDEEM RGDRLTQCLD DIMRASTEMM VQQQLKTIQL NSDVAAGFRK
     ALSKSLGDRV RAFHSILDGV ETTLTTASQY LDAVEEAAVK MKQWKQQQEE EQRRKHQAEL
     EKNKRQQEHD AATKAAAAQQ LMAQQSPVDL TAPTPTGLAS NLDKSNRVGG VKPFSAEFGH
     LDEMDLSMFG GIDGHGDFSL EDFNMGGNSV PLNGGRPRNM VMADAGGFPA RGGMGRGGAT
     AINNGAQEIK TSTNEASTNN RNNDGTGGAS NPRISSNYNS AVADHESIGL RVDANGQNTK
     QSGQQSGAGL NPDDYLTLND FNDLGIDWNT GENNELDLND FNI