ID   MED2_CANGA              Reviewed;         368 AA.
AC   Q6FWM4;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 2;
DE   AltName: Full=Mediator complex subunit 2;
GN   Name=MED2; OrderedLocusNames=CAGL0C04477g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 2 family.
CC       {ECO:0000305}.
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DR   EMBL; CR380949; CAG58276.1; -; Genomic_DNA.
DR   RefSeq; XP_445370.1; XM_445370.1.
DR   AlphaFoldDB; Q6FWM4; -.
DR   SMR; Q6FWM4; -.
DR   STRING; 5478.XP_445370.1; -.
DR   EnsemblFungi; CAG58276; CAG58276; CAGL0C04477g.
DR   GeneID; 2886897; -.
DR   KEGG; cgr:CAGL0C04477g; -.
DR   CGD; CAL0127500; MED2.
DR   VEuPathDB; FungiDB:CAGL0C04477g; -.
DR   eggNOG; ENOG502RZB6; Eukaryota.
DR   HOGENOM; CLU_046031_0_0_1; -.
DR   InParanoid; Q6FWM4; -.
DR   OMA; AEMMMQQ; -.
DR   Proteomes; UP000002428; Chromosome C.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IEA:EnsemblFungi.
DR   GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR021017; Mediator_Med2_fun.
DR   Pfam; PF11214; Med2; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..368
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   2"
FT                   /id="PRO_0000302014"
FT   REGION          112..231
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          265..308
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        112..162
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        168..212
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   368 AA;  41050 MW;  005801CA1E7706F9 CRC64;
     MSYKNRLTAC FDDILKVSAE MMMQQQLKNV QLDPYMVNGF SAQQQNTLKE KIHMFHGILD
     DLENMLSKST YYVDTLANLG KESKRQKELE LEKQREQEEE EKKQKLLELE RKKKEQEEEE
     EKKKKQKEEE EKRKKELEEQ ERKKKEQEEE EKRRRQQEQD GDKQQSMFDG LDFTNADLDT
     SQPGTSGQND IKSPTMGAGP QTAGTDKPNT ADGPDKTNPP IAAFGLGDSQ SGGLYNDLNT
     MDLSMFSELD GGGFDASGFD TANTSNANAT TNSVPNNNNP ATNDSNMNND PTAAINAFDG
     TAAGNNETLG QGEKLEFDQS NPSAMLGNDI NMGDNGEDYL TLNDFNDLNI DWSAAGEGGD
     LDLNGFNI