MED30_ARATH
ID MED30_ARATH Reviewed; 189 AA.
AC Q9FMV4; Q681J6;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 30;
GN Name=MED30; Synonyms=MED30_1; OrderedLocusNames=At5g63480;
GN ORFNames=MLE2.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA de los Reyes C., Quan R., Chen H., Bautista V., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 6-189.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [6]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the plant Mediator complex subunit 30 family.
CC {ECO:0000305}.
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DR EMBL; AB007649; BAB08813.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97757.1; -; Genomic_DNA.
DR EMBL; BT033074; ACE95179.1; -; mRNA.
DR EMBL; AK175621; BAD43384.1; -; mRNA.
DR RefSeq; NP_568973.1; NM_125743.4.
DR AlphaFoldDB; Q9FMV4; -.
DR SMR; Q9FMV4; -.
DR IntAct; Q9FMV4; 1.
DR STRING; 3702.AT5G63480.1; -.
DR iPTMnet; Q9FMV4; -.
DR PaxDb; Q9FMV4; -.
DR PRIDE; Q9FMV4; -.
DR ProteomicsDB; 228842; -.
DR EnsemblPlants; AT5G63480.1; AT5G63480.1; AT5G63480.
DR GeneID; 836467; -.
DR Gramene; AT5G63480.1; AT5G63480.1; AT5G63480.
DR KEGG; ath:AT5G63480; -.
DR Araport; AT5G63480; -.
DR TAIR; locus:2167321; AT5G63480.
DR eggNOG; ENOG502RY8Y; Eukaryota.
DR HOGENOM; CLU_130098_0_0_1; -.
DR InParanoid; Q9FMV4; -.
DR OrthoDB; 1527607at2759; -.
DR PhylomeDB; Q9FMV4; -.
DR PRO; PR:Q9FMV4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMV4; baseline and differential.
DR Genevisible; Q9FMV4; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR InterPro; IPR034568; MED30.
DR PANTHER; PTHR36406; PTHR36406; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..189
FT /note="Mediator of RNA polymerase II transcription subunit
FT 30"
FT /id="PRO_0000419193"
FT COILED 138..178
FT /evidence="ECO:0000255"
FT CONFLICT 93
FT /note="G -> E (in Ref. 4; BAD43384)"
FT /evidence="ECO:0000305"
FT CONFLICT 172
FT /note="K -> I (in Ref. 4; BAD43384)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 189 AA; 19814 MW; 23BCD525B18004C2 CRC64;
MLQKQVSTTT TMTTQELAME GEKQLEETIE AAFQIISAMN DELCNPSLWS TSATPSSAAT
TTGSNGSALV SADAAAIDGT SHHSESAGGG GGGGSGNSVL DEASLRYKNS VTSLRAVLAA
IPNSQKAKAS EMQNGLGSPE SEDEIEKLEE QALSLRMEIA KKNVHVKELI DKLRELIADI
STWQSPCSV
