ID   MED30_BOVIN             Reviewed;         193 AA.
AC   Q2YDF2;
DT   13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 30;
DE   AltName: Full=Mediator complex subunit 30;
DE   AltName: Full=Thyroid hormone receptor-associated protein 6;
DE   AltName: Full=Thyroid hormone receptor-associated protein complex 25 kDa component;
DE            Short=Trap25;
GN   Name=MED30; Synonyms=THRAP6, TRAP25;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC       MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC       MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC       MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC       CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC       module termed the CDK8 module. Mediator containing the CDK8 module is
CC       less active than Mediator lacking this module in supporting
CC       transcriptional activation. Individual preparations of the Mediator
CC       complex lacking one or more distinct subunits have been variously
CC       termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 30 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC110250; AAI10251.1; -; mRNA.
DR   RefSeq; NP_001073086.1; NM_001079618.2.
DR   AlphaFoldDB; Q2YDF2; -.
DR   SMR; Q2YDF2; -.
DR   STRING; 9913.ENSBTAP00000009150; -.
DR   PaxDb; Q2YDF2; -.
DR   Ensembl; ENSBTAT00000084060; ENSBTAP00000073342; ENSBTAG00000026254.
DR   GeneID; 613879; -.
DR   KEGG; bta:613879; -.
DR   CTD; 90390; -.
DR   VEuPathDB; HostDB:ENSBTAG00000026254; -.
DR   VGNC; VGNC:31366; MED30.
DR   eggNOG; ENOG502QV3C; Eukaryota.
DR   GeneTree; ENSGT00390000010887; -.
DR   HOGENOM; CLU_074190_1_1_1; -.
DR   InParanoid; Q2YDF2; -.
DR   OrthoDB; 1549016at2759; -.
DR   TreeFam; TF324588; -.
DR   Proteomes; UP000009136; Chromosome 14.
DR   Bgee; ENSBTAG00000026254; Expressed in oocyte and 106 other tissues.
DR   ExpressionAtlas; Q2YDF2; baseline.
DR   GO; GO:0070847; C:core mediator complex; IEA:Ensembl.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0046966; F:nuclear thyroid hormone receptor binding; IEA:Ensembl.
DR   GO; GO:0003713; F:transcription coactivator activity; IEA:Ensembl.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:Ensembl.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0035019; P:somatic stem cell population maintenance; IEA:Ensembl.
DR   InterPro; IPR021019; Mediator_Med30_met.
DR   PANTHER; PTHR31705; PTHR31705; 1.
DR   Pfam; PF11315; Med30; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Activator; Coiled coil; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q96HR3"
FT   CHAIN           2..193
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   30"
FT                   /id="PRO_0000239405"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          71..93
FT                   /evidence="ECO:0000255"
FT   MOD_RES         2
FT                   /note="N-acetylserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96HR3"
SQ   SEQUENCE   193 AA;  21691 MW;  9CE39BBEB1487A59 CRC64;
     MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME IFQLLRNMQL
     PNGVTYHTGT YQDRLAKLQD HLRQLSILFR KLRLVYDKCN ENCGGMDPIP VEQLIPYVEE
     DGSKDDRAGP PRFASEERRE IAEVNKFQVN SKGTQPYLHM YPFSSKLPSH SGCRIPLSRI
     PCAVQNSNRR INS