ID   MED30_DROPS             Reviewed;         325 AA.
AC   Q29DV9;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 2.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 30;
DE   AltName: Full=Mediator complex subunit 30;
GN   Name=MED30; ORFNames=GA14371;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25 / Tucson 14011-0121.94;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA   Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA   Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA   Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA   Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA   Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA   Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT   gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 30 family.
CC       {ECO:0000305}.
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DR   EMBL; CH379070; EAL30304.2; -; Genomic_DNA.
DR   RefSeq; XP_001352804.2; XM_001352768.3.
DR   AlphaFoldDB; Q29DV9; -.
DR   SMR; Q29DV9; -.
DR   STRING; 7237.FBpp0286924; -.
DR   PRIDE; Q29DV9; -.
DR   EnsemblMetazoa; FBtr0288486; FBpp0286924; FBgn0074399.
DR   GeneID; 4812005; -.
DR   KEGG; dpo:Dpse_GA14371; -.
DR   eggNOG; ENOG502QV3C; Eukaryota.
DR   HOGENOM; CLU_074190_0_0_1; -.
DR   InParanoid; Q29DV9; -.
DR   OMA; YFRTIRL; -.
DR   Proteomes; UP000001819; Chromosome X.
DR   Bgee; FBgn0074399; Expressed in female reproductive system and 3 other tissues.
DR   GO; GO:0016592; C:mediator complex; ISS:UniProtKB.
DR   GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   InterPro; IPR021019; Mediator_Med30_met.
DR   PANTHER; PTHR31705; PTHR31705; 1.
DR   Pfam; PF11315; Med30; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..325
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   30"
FT                   /id="PRO_0000305911"
FT   REGION          1..26
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          109..176
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        109..159
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   325 AA;  36309 MW;  771F5C4FB11DEA07 CRC64;
     MWKYGQNQGN QGPAGGGGGA PNMMPMGGFM QGGGGMQGGG GMQHGNMPQM HMTPQQQQQQ
     QQMNMMGGPG GMQMNPNAVG PTGLMPGMSP QHQMQQQQQM MQGVPMSMPP QQAMQQQMMG
     PQQGLGMGGS SGPQQQQQLQ QQQQQSNLQQ QQQQHNVGSG GAPGAGGVGN NMLAISQPNP
     HKEINIVQLS RLGQETVQDI ASRFQEVFSA LKNIQPTSHR DNNTEKKVQE YFRTIRLLFK
     RVRIIYDKCN DAVMDYMNAE SLIPYKDEPE PRIETSQCDE YRKVLQENHE YIETVKLKNR
     QLREIIDRTR IIIWEINTML AMRRS