MED31_ARATH
ID MED31_ARATH Reviewed; 196 AA.
AC Q8VYB1; F4K2N8;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 31;
DE AltName: Full=Mediator complex subunit 31;
DE AltName: Full=Mediator complex subunit SOH1;
GN Name=MED31; Synonyms=MED31_1; OrderedLocusNames=At5g19910;
GN ORFNames=F28I16.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [5]
RP IDENTIFICATION, SUBUNIT, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:22021418}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VYB1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VYB1-2; Sequence=VSP_043983;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 31 family.
CC {ECO:0000305}.
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DR EMBL; AF296836; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92764.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92765.1; -; Genomic_DNA.
DR EMBL; AY072222; AAL60043.1; -; mRNA.
DR EMBL; BT002744; AAO22573.1; -; mRNA.
DR RefSeq; NP_001190342.1; NM_001203413.1. [Q8VYB1-2]
DR RefSeq; NP_197491.2; NM_121998.5. [Q8VYB1-1]
DR AlphaFoldDB; Q8VYB1; -.
DR SMR; Q8VYB1; -.
DR BioGRID; 17389; 1.
DR IntAct; Q8VYB1; 5.
DR PRIDE; Q8VYB1; -.
DR ProteomicsDB; 228843; -. [Q8VYB1-1]
DR EnsemblPlants; AT5G19910.1; AT5G19910.1; AT5G19910. [Q8VYB1-1]
DR EnsemblPlants; AT5G19910.2; AT5G19910.2; AT5G19910. [Q8VYB1-2]
DR GeneID; 832113; -.
DR Gramene; AT5G19910.1; AT5G19910.1; AT5G19910. [Q8VYB1-1]
DR Gramene; AT5G19910.2; AT5G19910.2; AT5G19910. [Q8VYB1-2]
DR KEGG; ath:AT5G19910; -.
DR Araport; AT5G19910; -.
DR TAIR; locus:2147675; AT5G19910.
DR InParanoid; Q8VYB1; -.
DR OMA; KNDMRNS; -.
DR OrthoDB; 1480492at2759; -.
DR PhylomeDB; Q8VYB1; -.
DR PRO; PR:Q8VYB1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VYB1; baseline and differential.
DR Genevisible; Q8VYB1; AT.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.1340; -; 1.
DR InterPro; IPR038089; Med31_sf.
DR InterPro; IPR008831; Mediator_Med31.
DR PANTHER; PTHR13186; PTHR13186; 1.
DR Pfam; PF05669; Med31; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..196
FT /note="Mediator of RNA polymerase II transcription subunit
FT 31"
FT /id="PRO_0000212532"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 133..196
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..151
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 152..182
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 108
FT /note="K -> KWFKMLGYWCFWNVFWGLVEFRVSLVQNLAY (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_043983"
SQ SEQUENCE 196 AA; 22819 MW; 706E3EB75C8F0A19 CRC64;
MASPEEMGDD ASEIPSPPKN TYKDPDGGRQ RFLLELEFIQ CLANPTYIHY LAQNRYFEDE
AFIGYLKYLQ YWQRPEYIKF IMYPHCLYFL ELLQNPNFRT AMAHPANKEL AHRQQFYYWK
NYRNNRLKHI LPRPLPEPVP PQPPVAPSTS LPPAPSATAA LSPALSPMQY NNMLSKNDTR
NMGATGIDRR KRKKGI
