MED32_ARATH
ID MED32_ARATH Reviewed; 151 AA.
AC Q84VW5; Q9SAA1;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 32;
DE AltName: Full=Mediator of RNA polymerase II transcription subunit 2;
GN Name=MED32; Synonyms=MED2, MED29, MED2_1; OrderedLocusNames=At1g11760;
GN ORFNames=F25C20.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, INTERACTION WITH MED6, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [6]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [7]
RP SUBUNIT, MUTAGENESIS OF CYS-55 AND CYS-73, AND INTERACTION WITH GEBPL.
RX PubMed=25877331; DOI=10.1042/bj20150132;
RA Shaikhali J., Davoine C., Braennstroem K., Rouhier N., Bygdell J.,
RA Bjoerklund S., Wingsle G.;
RT "Biochemical and redox characterization of the mediator complex and its
RT associated transcription factor GeBPL, a GLABROUS1 enhancer binding
RT protein.";
RL Biochem. J. 468:385-400(2015).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional pre-initiation complex with RNA polymerase II and the
CC general transcription factors. {ECO:0000305|PubMed:17560376,
CC ECO:0000305|PubMed:22021418}.
CC -!- SUBUNIT: Oligomers (PubMed:25877331). Component of the Mediator complex
CC (PubMed:17560376). Interacts with MED6 (PubMed:17560376). Interacts
CC with GEBPL (PubMed:25877331). {ECO:0000269|PubMed:17560376,
CC ECO:0000269|PubMed:25877331}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the mediator complex subunit 32 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD30247.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007296; AAD30247.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE28781.1; -; Genomic_DNA.
DR EMBL; AK228068; BAF00028.1; -; mRNA.
DR EMBL; BT004763; AAO44029.1; -; mRNA.
DR PIR; D86251; D86251.
DR RefSeq; NP_172641.2; NM_101048.4.
DR AlphaFoldDB; Q84VW5; -.
DR SMR; Q84VW5; -.
DR BioGRID; 22960; 3.
DR IntAct; Q84VW5; 8.
DR STRING; 3702.AT1G11760.1; -.
DR iPTMnet; Q84VW5; -.
DR PaxDb; Q84VW5; -.
DR PRIDE; Q84VW5; -.
DR ProteomicsDB; 228852; -.
DR EnsemblPlants; AT1G11760.1; AT1G11760.1; AT1G11760.
DR GeneID; 837720; -.
DR Gramene; AT1G11760.1; AT1G11760.1; AT1G11760.
DR KEGG; ath:AT1G11760; -.
DR Araport; AT1G11760; -.
DR TAIR; locus:2027377; AT1G11760.
DR eggNOG; ENOG502RXGF; Eukaryota.
DR HOGENOM; CLU_112745_0_0_1; -.
DR InParanoid; Q84VW5; -.
DR OMA; FKQHWEL; -.
DR OrthoDB; 1564416at2759; -.
DR PhylomeDB; Q84VW5; -.
DR PRO; PR:Q84VW5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84VW5; baseline and differential.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IDA:TAIR.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; IDA:TAIR.
DR GO; GO:0009631; P:cold acclimation; IMP:TAIR.
DR GO; GO:0010150; P:leaf senescence; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:TAIR.
DR GO; GO:0042542; P:response to hydrogen peroxide; IMP:TAIR.
DR GO; GO:0048364; P:root development; IMP:TAIR.
DR InterPro; IPR033244; MED32.
DR PANTHER; PTHR35989; PTHR35989; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..151
FT /note="Mediator of RNA polymerase II transcription subunit
FT 32"
FT /id="PRO_0000418116"
FT REGION 128..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 130..151
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 55
FT /note="C->S: Loss of oligomerization."
FT /evidence="ECO:0000269|PubMed:25877331"
FT MUTAGEN 73
FT /note="C->S: Loss of oligomerization."
FT /evidence="ECO:0000269|PubMed:25877331"
SQ SEQUENCE 151 AA; 16013 MW; 9D2B07AECF1C9F30 CRC64;
MDNIVDSLNK AYEKFVLASA GVLESKESAG GQKALLTDTA LENFKEKWEL FRVACDQAEE
FVESVKQRIG SECLVDEATG LTTTAAGGQA PAAVTGAATS LPPISAVRLE QMSRAVRWLV
LELQRGSGVA PGSVHSSSTG FDSRFSEDST Q
