ID   MED3_ASHGO              Reviewed;         332 AA.
AC   Q752T7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 3;
DE   AltName: Full=Mediator complex subunit 3;
GN   Name=PGD1; Synonyms=MED3; OrderedLocusNames=AFR486C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 25 AND 270.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       regulated gene transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 3 family.
CC       {ECO:0000305}.
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DR   EMBL; AE016819; AAS53857.2; -; Genomic_DNA.
DR   RefSeq; NP_986033.2; NM_212169.2.
DR   AlphaFoldDB; Q752T7; -.
DR   SMR; Q752T7; -.
DR   STRING; 33169.AAS53857; -.
DR   EnsemblFungi; AAS53857; AAS53857; AGOS_AFR486C.
DR   GeneID; 4622312; -.
DR   KEGG; ago:AGOS_AFR486C; -.
DR   eggNOG; ENOG502S3GT; Eukaryota.
DR   HOGENOM; CLU_049224_0_0_1; -.
DR   InParanoid; Q752T7; -.
DR   OMA; FIQIMAN; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR020998; Med3.
DR   Pfam; PF11593; Med3; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..332
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   3"
FT                   /id="PRO_0000302016"
FT   REGION          125..206
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          221..242
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        135..156
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        179..202
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   332 AA;  35772 MW;  1D90C31975EB2F3F CRC64;
     MGSNIDGILR ENLTLEELQE WLTSSEGNKL AIDDHIKATQ GRVLPLRLLF NEFLRTISHI
     EQLSDKTPQE KFQLIRSKLQ ELYGKLHALV RDFQRLQPLF DTMVPFSETS ERKFYPKETL
     GTAVEPVRPL ASPSYRRPSN RSSADTPSSN APTPSAAVVS GAALVAPQVK HQRRPRTNTA
     KRQPSVSASV VPSANSSGPA TIPGATPLML SGMSPLNMVA SPLNGISPSR KPAQPHHQTT
     PSAALGMQPM QQKQMSIQAK ATPSKSGTIS SANLTPQSIL NMSAFENSAG GVPNSAVPLN
     QNNNAIMGFP TDIDNIDLNA LELGSLNMDL LG