ID   MED3_KLULA              Reviewed;         318 AA.
AC   Q6CNQ9;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 3;
DE   AltName: Full=Mediator complex subunit 3;
GN   Name=PGD1; Synonyms=MED3; OrderedLocusNames=KLLA0E10615g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       regulated gene transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 3 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR382125; CAG99517.1; -; Genomic_DNA.
DR   RefSeq; XP_454430.1; XM_454430.1.
DR   AlphaFoldDB; Q6CNQ9; -.
DR   SMR; Q6CNQ9; -.
DR   STRING; 28985.XP_454430.1; -.
DR   EnsemblFungi; CAG99517; CAG99517; KLLA0_E10627g.
DR   GeneID; 2893658; -.
DR   KEGG; kla:KLLA0_E10627g; -.
DR   eggNOG; ENOG502S3GT; Eukaryota.
DR   HOGENOM; CLU_049224_0_0_1; -.
DR   InParanoid; Q6CNQ9; -.
DR   OMA; FIQIMAN; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR020998; Med3.
DR   Pfam; PF11593; Med3; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..318
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   3"
FT                   /id="PRO_0000302018"
FT   REGION          134..225
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        134..159
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        171..225
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   318 AA;  34267 MW;  19A0A0D0E10E3601 CRC64;
     MAVGPPDQIF KDNLTFDTFR ELIIEKESTA DEVADKVLEA KKALLPIRTL MTEFVGMIAN
     LESMGNKTSQ EKFLAIRMKL IELQNNIQKF SKDFQQLQPV MRTMDKFNEE VNAGEKKFFV
     QETLGYTQLA SNGSAAGITK TSSGNDGNTT GSTANTMAMA KGLKKNAAGK PNTGTGVQSG
     PGRRNSTKKT GHTGPATAPT TSNSAASAAA AAANTPSLKQ IPNTQPMQLM PGVSPMAMAS
     PLNNISPQRK LTQHVNQSRE NSLHQGVTPS ASMITPQNIL NMSAFDLNQN QTPQSLDNVN
     NMDLTNLDLD SLNMEFLN