ID   MED4_ARATH              Reviewed;         426 AA.
AC   Q9LZ00; Q7Y210;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
GN   Name=MED4; Synonyms=MED4_1; OrderedLocusNames=At5g02850;
GN   ORFNames=F9G14.160;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Yamada K., Dale J.M., Hsuan V.W., Onodera C.S., Quach H., Toriumi M.,
RA   Wong C., Wu H.C., Yu G., Yuan S., Carninci P., Chen H., Cheuk R.,
RA   Hayashizaki Y., Ishida J., Jones T., Kamiya A., Kawai J., Kim C.J.,
RA   Narusaka M., Nguyen M., Palm C.J., Sakurai T., Satou M., Seki M., Shinn P.,
RA   Southwick A., Tripp M.G., Wu T., Shinozaki K., Davis R.W., Ecker J.R.,
RA   Theologis A.;
RT   "Arabidopsis full length cDNA clones.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP   COMPLEX, AND NOMENCLATURE.
RX   PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA   Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT   "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT   as the Med25 subunit.";
RL   Mol. Cell 26:717-729(2007).
RN   [5]
RP   IDENTIFICATION, IDENTIFICATION IN THE MEDIATOR COMPLEX, AND NOMENCLATURE.
RX   PubMed=22021418; DOI=10.1104/pp.111.188300;
RA   Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT   "The Mediator complex in plants: structure, phylogeny, and expression
RT   profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT   (rice) during reproduction and abiotic stress.";
RL   Plant Physiol. 157:1609-1627(2011).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19376835; DOI=10.1104/pp.109.138677;
RA   Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA   Grossmann J., Gruissem W., Baginsky S.;
RT   "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT   chloroplast kinase substrates and phosphorylation networks.";
RL   Plant Physiol. 150:889-903(2009).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. The Mediator complex, having a compact
CC       conformation in its free form, is recruited to promoters by direct
CC       interactions with regulatory proteins and serves for the assembly of a
CC       functional preinitiation complex with RNA polymerase II and the general
CC       transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex.
CC       {ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:22021418}.
CC   -!- INTERACTION:
CC       Q9LZ00; Q8RWA2: MED9; NbExp=5; IntAct=EBI-1386214, EBI-1386224;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
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DR   EMBL; AL162973; CAB86039.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90527.1; -; Genomic_DNA.
DR   EMBL; BT008646; AAP40461.1; -; mRNA.
DR   PIR; T48306; T48306.
DR   RefSeq; NP_195905.1; NM_120363.3.
DR   AlphaFoldDB; Q9LZ00; -.
DR   SMR; Q9LZ00; -.
DR   BioGRID; 17038; 3.
DR   IntAct; Q9LZ00; 91.
DR   STRING; 3702.AT5G02850.1; -.
DR   iPTMnet; Q9LZ00; -.
DR   PaxDb; Q9LZ00; -.
DR   PRIDE; Q9LZ00; -.
DR   ProteomicsDB; 238944; -.
DR   EnsemblPlants; AT5G02850.1; AT5G02850.1; AT5G02850.
DR   GeneID; 831762; -.
DR   Gramene; AT5G02850.1; AT5G02850.1; AT5G02850.
DR   KEGG; ath:AT5G02850; -.
DR   Araport; AT5G02850; -.
DR   TAIR; locus:2151266; AT5G02850.
DR   eggNOG; ENOG502QQ6I; Eukaryota.
DR   HOGENOM; CLU_057409_1_0_1; -.
DR   InParanoid; Q9LZ00; -.
DR   OMA; MMQSHLP; -.
DR   OrthoDB; 1404352at2759; -.
DR   PhylomeDB; Q9LZ00; -.
DR   PRO; PR:Q9LZ00; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LZ00; baseline and differential.
DR   Genevisible; Q9LZ00; AT.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..426
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   4"
FT                   /id="PRO_0000418110"
FT   REGION          1..56
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          214..236
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          373..426
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          160..212
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        18..56
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        408..426
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        34
FT                   /note="P -> T (in Ref. 3; AAP40461)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   426 AA;  46156 MW;  4AC6A38586479FCE CRC64;
     MLQHQIVQSP ARLGLTGPGS PSVQNPTPTR HGHPTSSSSS QSQHQQIQQQ PNLLPSSTVA
     AASSASASAA VSSSALLSLL PPLPRAQALL QQMAVLTSKL FDVSPNRAIW LSAFRGSLPS
     FLSSHSLPPP PPLENPSPSS TKEILSQFNS LQTQLFEAVT ELQEILDLQD AKQKVAREIK
     SKDSSLLAFA NKLKDAERVL DMLVDDYSDY RKPKRSKIEE DDEDNDNESS SSSTTVSSQL
     KLKDILAYAH KISYTTFAPP EFGAGQAPLR GALPPAPQDE QMRASQLYTF ADLDIGLPKT
     VENMEKKVEA LIEPPPPPEA MDISAIHNLL PPNIAVPSGW KPGMPVELPR DLPLPPPGWK
     PGDPVVLPPL ESIAAPRAED HQHMRPSQGL HRPPDVIQVR AVQLDILEDD DSSDYSSDDA
     SSDDED