ID   MED4_CAEEL              Reviewed;         334 AA.
AC   Q23523;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 2.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
DE   AltName: Full=Mediator complex subunit 4;
GN   Name=mdt-4; ORFNames=ZK546.13;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
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DR   EMBL; FO081669; CCD73223.1; -; Genomic_DNA.
DR   PIR; T27901; T27901.
DR   RefSeq; NP_494905.2; NM_062504.4.
DR   AlphaFoldDB; Q23523; -.
DR   SMR; Q23523; -.
DR   IntAct; Q23523; 1.
DR   STRING; 6239.ZK546.13; -.
DR   iPTMnet; Q23523; -.
DR   EPD; Q23523; -.
DR   PaxDb; Q23523; -.
DR   PeptideAtlas; Q23523; -.
DR   EnsemblMetazoa; ZK546.13.1; ZK546.13.1; WBGene00007012.
DR   GeneID; 173852; -.
DR   KEGG; cel:CELE_ZK546.13; -.
DR   UCSC; ZK546.13; c. elegans.
DR   CTD; 173852; -.
DR   WormBase; ZK546.13; CE34460; WBGene00007012; mdt-4.
DR   eggNOG; KOG4552; Eukaryota.
DR   GeneTree; ENSGT00390000012063; -.
DR   HOGENOM; CLU_832182_0_0_1; -.
DR   InParanoid; Q23523; -.
DR   OMA; TPNEKQW; -.
DR   OrthoDB; 1333917at2759; -.
DR   PhylomeDB; Q23523; -.
DR   PRO; PR:Q23523; -.
DR   Proteomes; UP000001940; Chromosome II.
DR   Bgee; WBGene00007012; Expressed in embryo and 3 other tissues.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..334
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   4"
FT                   /id="PRO_0000302071"
FT   REGION          193..334
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          71..100
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        193..227
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        244..286
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        304..320
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   334 AA;  36662 MW;  984E74FA618D20DE CRC64;
     MAETDERSLR DLLLESADDL EHIVKMIVDT LINRDKSVML KNGETVTNII KLFDSKQDSI
     KTLLKRVPEF QEREQLIRTL EAHVEKRDEV IQQLETNLKS CEVALTRSCF HANQKLKQMK
     EAELRPVNSE MLIKLAHQIS KHNSVSAPLT WQMGDPSRPF PQEHEFRAGH LLNSKVQSTG
     PQLLPGKNVA QRPLITSPSA SSSNGGTAPI RTVGTPLINS APTGDFSPRT GFGTDEPPPI
     QQQVLRGATP NEKQWQNPGV SGATSTQSPY NRVSQSPSSS PNVKLKITGL PNRPGGIDQV
     QDVRDVEQMS SDSSNSSDSS DDEGASKKTG SSNK