ID   MED4_CANAL              Reviewed;         323 AA.
AC   Q59U73; A0A1D8PHX4;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 2.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
DE   AltName: Full=Mediator complex subunit 4;
GN   Name=MED4; OrderedLocusNames=CAALFM_C207500CA;
GN   ORFNames=CaO19.1878, CaO19.9434;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP017624; AOW27748.1; -; Genomic_DNA.
DR   RefSeq; XP_713135.2; XM_708042.2.
DR   AlphaFoldDB; Q59U73; -.
DR   SMR; Q59U73; -.
DR   BioGRID; 1228243; 2.
DR   STRING; 237561.Q59U73; -.
DR   PRIDE; Q59U73; -.
DR   GeneID; 3645209; -.
DR   KEGG; cal:CAALFM_C207500CA; -.
DR   CGD; CAL0000181311; MED4.
DR   VEuPathDB; FungiDB:C2_07500C_A; -.
DR   eggNOG; ENOG502RXM0; Eukaryota.
DR   HOGENOM; CLU_071875_0_0_1; -.
DR   InParanoid; Q59U73; -.
DR   OrthoDB; 1527310at2759; -.
DR   PRO; PR:Q59U73; -.
DR   Proteomes; UP000000559; Chromosome 2.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..323
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   4"
FT                   /id="PRO_0000302073"
FT   REGION          1..36
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          241..323
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          79..167
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        14..36
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        241..261
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        274..306
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   323 AA;  37008 MW;  C78914A861ECDFF1 CRC64;
     MLPFKKADSP FKSNPVSRVG SSTRLNQLGN IKSNPTTPNA ALYVTSSLNP SKNLPTNAAN
     VKSRLQTQKD LDAFKQLPMV QKVNEYERLL NELSEAVSQF KNDELQEKIG QIITCNDVLK
     QQIEDLNKHR NYSYEVDKLS DRNKILEENS KFILKELVSY RNELKKLPKL PKSDKMVNRN
     VDVDDILKYA FKLAKFTKAP ATVANMPFQI HPNNYVWPAE DSLRRGMLAQ ASLQAEEIIR
     HELGETDKEN SNEVKTESKV DHDDDDDDEM EDVRISNENT NDEQRSKPPA ASEHDTSKRK
     EEQNQQPVDL NLDLFDPDDE YSD