ID   MED4_CRYNJ              Reviewed;         234 AA.
AC   P0CO72; Q55QP0; Q5KFH5;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
DE   AltName: Full=Mediator complex subunit 4;
GN   Name=MED4; OrderedLocusNames=CNF01730;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
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DR   EMBL; AE017346; AAW44207.1; -; Genomic_DNA.
DR   RefSeq; XP_571514.1; XM_571514.1.
DR   AlphaFoldDB; P0CO72; -.
DR   SMR; P0CO72; -.
DR   STRING; 5207.AAW44207; -.
DR   PaxDb; P0CO72; -.
DR   EnsemblFungi; AAW44207; AAW44207; CNF01730.
DR   GeneID; 3258430; -.
DR   KEGG; cne:CNF01730; -.
DR   VEuPathDB; FungiDB:CNF01730; -.
DR   eggNOG; ENOG502SDCP; Eukaryota.
DR   HOGENOM; CLU_1200540_0_0_1; -.
DR   InParanoid; P0CO72; -.
DR   OMA; ICMELAN; -.
DR   OrthoDB; 1549398at2759; -.
DR   Proteomes; UP000002149; Chromosome 6.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..234
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   4"
FT                   /id="PRO_0000302076"
FT   REGION          188..234
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          71..124
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        192..218
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   234 AA;  26249 MW;  7556F89651E12AAB CRC64;
     MAHTTPRSHP PPHSLLQNLT TQSLLLSHLF TLIASPPNPN TSTQTQLNQV YSALQLSTLD
     LSGLVKEVGH HQEAYRRLVE KKNEVAGLEM RVRGLVKRLE EGRKELEGMI DQGERSLEDI
     EKSEREPVPA KTLMAHAQSL SKHSSAPVSS LLAPVDKAQY APWPTEMSMR MGLLFQLEGS
     MSGMGERGVV GEEQKAPQKV EERREHVEHE ESDRRYDPNA VFQLDLNSDE SDED