ID   MED4_DANRE              Reviewed;         254 AA.
AC   Q561X3;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
DE   AltName: Full=Mediator complex subunit 4;
GN   Name=med4; ORFNames=zgc:110646;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
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DR   EMBL; BC092965; AAH92965.1; -; mRNA.
DR   RefSeq; NP_001017883.1; NM_001017883.1.
DR   AlphaFoldDB; Q561X3; -.
DR   SMR; Q561X3; -.
DR   STRING; 7955.ENSDARP00000060839; -.
DR   PaxDb; Q561X3; -.
DR   GeneID; 550582; -.
DR   KEGG; dre:550582; -.
DR   CTD; 29079; -.
DR   ZFIN; ZDB-GENE-050417-438; med4.
DR   eggNOG; KOG4552; Eukaryota.
DR   InParanoid; Q561X3; -.
DR   OrthoDB; 1333917at2759; -.
DR   PhylomeDB; Q561X3; -.
DR   Reactome; R-DRE-212436; Generic Transcription Pathway.
DR   PRO; PR:Q561X3; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   2: Evidence at transcript level;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..254
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   4"
FT                   /id="PRO_0000302065"
FT   REGION          215..254
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          72..114
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        226..240
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   254 AA;  28053 MW;  EB97AE48259A61D5 CRC64;
     MAAAAADKST KERLLSTLDD LEVLSRELIE MLALSRSQKL PPPGEDTLIL ELLIQRDKEF
     QELMQTALEQ GRVHQEMQSL EKEVEKRDSD IQQLQKQLKE AEHILATAVY QAKEKLKSID
     KARKGSISSK EIIKYAHRIS ASNAVCAPLN WVPGDPRRPY PTDLEMRSGM LGNMSNMSTN
     GVNGHLPGDA LAAGRLPDVL TPQYPWQSTD VSMGILPPHH GNDFGLEPPG HNKENEDDVE
     AMSTDSSSSS SDSD