ID   MED4_DEBHA              Reviewed;         322 AA.
AC   Q6BKB5;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
DE   AltName: Full=Mediator complex subunit 4;
GN   Name=MED4; OrderedLocusNames=DEHA2F23320g;
OS   Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990
OS   / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX   NCBI_TaxID=284592;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAG89762.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CR382138; CAG89762.2; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_461356.2; XM_461356.2.
DR   AlphaFoldDB; Q6BKB5; -.
DR   SMR; Q6BKB5; -.
DR   STRING; 4959.XP_461356.2; -.
DR   EnsemblFungi; CAG89762; CAG89762; DEHA2F23320g.
DR   GeneID; 2903174; -.
DR   KEGG; dha:DEHA2F23320g; -.
DR   eggNOG; ENOG502RXM0; Eukaryota.
DR   HOGENOM; CLU_071875_0_0_1; -.
DR   InParanoid; Q6BKB5; -.
DR   OrthoDB; 1527310at2759; -.
DR   Proteomes; UP000000599; Chromosome F.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..322
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   4"
FT                   /id="PRO_0000302077"
FT   REGION          274..322
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        291..307
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   322 AA;  35510 MW;  59E144DC5C97489C CRC64;
     MIPHKKNDSS FLSVPVSRVG STQRLNQLAS HGVATGSPNA YSSIPSTPSA YVSSSLNPTK
     GISAGPESSN QIITKEDRDA FGKLSIVEKV NEFEQNLTNL SFKVSSFKED ELASLIGKLI
     ELNDIICSEI GELKKHRDLG ILIKGLKDDQ AHLDNTSKII LKELITYRNE LKKLPRLPSN
     KNKASVFPSP NDSKVDVNEV LKYASKLSKF TKAPPTVSNL PFQIHPNNYV WPAEDALRRG
     MLAMSSIKVD DIINAELGED TLEVKEEAIK KVEDNKVESI TNSPPAERRG SFGERTGESR
     KQESQGALDL DLFDPDEEDF SD