ID   MED4_DICDI              Reviewed;         343 AA.
AC   Q54D00;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Putative mediator of RNA polymerase II transcription subunit 4;
DE   AltName: Full=Putative mediator complex subunit 4;
GN   Name=med4; ORFNames=DDB_G0292608;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=18515835; DOI=10.1093/nar/gkn349;
RA   Bourbon H.-M.;
RT   "Comparative genomics supports a deep evolutionary origin for the large,
RT   four-module transcriptional mediator complex.";
RL   Nucleic Acids Res. 36:3993-4008(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000194; EAL61107.1; -; Genomic_DNA.
DR   RefSeq; XP_629520.1; XM_629518.1.
DR   AlphaFoldDB; Q54D00; -.
DR   SMR; Q54D00; -.
DR   STRING; 44689.DDB0266848; -.
DR   PaxDb; Q54D00; -.
DR   EnsemblProtists; EAL61107; EAL61107; DDB_G0292608.
DR   GeneID; 8628772; -.
DR   KEGG; ddi:DDB_G0292608; -.
DR   dictyBase; DDB_G0292608; med4.
DR   eggNOG; ENOG502RE10; Eukaryota.
DR   HOGENOM; CLU_809943_0_0_1; -.
DR   InParanoid; Q54D00; -.
DR   OMA; YAHKISG; -.
DR   PRO; PR:Q54D00; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; ISS:dictyBase.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..343
FT                   /note="Putative mediator of RNA polymerase II transcription
FT                   subunit 4"
FT                   /id="PRO_0000388644"
FT   REGION          247..343
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          86..125
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        247..317
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        320..343
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   343 AA;  38449 MW;  3BF62E6A6FE76BF5 CRC64;
     MNKRINEVSG RPIGEYLSRD ISDFQRVSRT LFECFSSLAT GATLNSQQLQ QIQLPIDQIS
     NNATPNMVLQ LMNHLIDVDK IYQNTLKKLE KHQKIQKEIT EIQKEIEEKD KLISTLALNL
     KDIESFLENE LSQDNVNINN GNASDGIVNE VLIDLKQPDD AASMDNIVNR EVSPEDLISY
     AHKISGTTSA PFGYQPNAPL ASLFKPPAPQ DEMMRSSVLF TKPPPHVLKY YGLAEIDVTT
     PTMAANISSP FSIGGNVGDV TTPTSKEQED QQQQQQQQQP QQQLSQSQQS QQQTESELQP
     IQSILQPPQQ LNIDLDLNPD LDSSGDDDDE DDDDEESEEV EWD