MED4_MOUSE
ID MED4_MOUSE Reviewed; 270 AA.
AC Q9CQA5; Q3UL96; Q8BVG6;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
DE AltName: Full=Mediator complex subunit 4;
GN Name=Med4; Synonyms=Vdrip;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Olfactory bulb;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CQA5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CQA5-2; Sequence=VSP_027918, VSP_027919;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE26554.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK010270; BAB26809.1; -; mRNA.
DR EMBL; AK010686; BAB27118.1; -; mRNA.
DR EMBL; AK078288; BAC37206.1; -; mRNA.
DR EMBL; AK145636; BAE26554.1; ALT_INIT; mRNA.
DR EMBL; BC024950; AAH24950.1; -; mRNA.
DR CCDS; CCDS49538.1; -. [Q9CQA5-1]
DR RefSeq; NP_080395.1; NM_026119.3. [Q9CQA5-1]
DR PDB; 6W1S; EM; 4.02 A; B=30-129.
DR PDBsum; 6W1S; -.
DR AlphaFoldDB; Q9CQA5; -.
DR SMR; Q9CQA5; -.
DR BioGRID; 212147; 3.
DR ComplexPortal; CPX-3264; Core mediator complex.
DR CORUM; Q9CQA5; -.
DR IntAct; Q9CQA5; 5.
DR MINT; Q9CQA5; -.
DR STRING; 10090.ENSMUSP00000022705; -.
DR iPTMnet; Q9CQA5; -.
DR PhosphoSitePlus; Q9CQA5; -.
DR EPD; Q9CQA5; -.
DR MaxQB; Q9CQA5; -.
DR PaxDb; Q9CQA5; -.
DR PeptideAtlas; Q9CQA5; -.
DR PRIDE; Q9CQA5; -.
DR ProteomicsDB; 292208; -. [Q9CQA5-1]
DR ProteomicsDB; 292209; -. [Q9CQA5-2]
DR Antibodypedia; 1791; 219 antibodies from 34 providers.
DR Ensembl; ENSMUST00000022705; ENSMUSP00000022705; ENSMUSG00000022109. [Q9CQA5-1]
DR GeneID; 67381; -.
DR KEGG; mmu:67381; -.
DR UCSC; uc007upu.1; mouse. [Q9CQA5-1]
DR CTD; 29079; -.
DR MGI; MGI:1914631; Med4.
DR VEuPathDB; HostDB:ENSMUSG00000022109; -.
DR eggNOG; KOG4552; Eukaryota.
DR GeneTree; ENSGT00390000012063; -.
DR HOGENOM; CLU_082233_0_0_1; -.
DR InParanoid; Q9CQA5; -.
DR OMA; GEIHMSV; -.
DR OrthoDB; 1333917at2759; -.
DR PhylomeDB; Q9CQA5; -.
DR TreeFam; TF324421; -.
DR Reactome; R-MMU-212436; Generic Transcription Pathway.
DR BioGRID-ORCS; 67381; 23 hits in 73 CRISPR screens.
DR ChiTaRS; Med4; mouse.
DR PRO; PR:Q9CQA5; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q9CQA5; protein.
DR Bgee; ENSMUSG00000022109; Expressed in dorsal pancreas and 253 other tissues.
DR Genevisible; Q9CQA5; MM.
DR GO; GO:0070847; C:core mediator complex; ISO:MGI.
DR GO; GO:0016592; C:mediator complex; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0046966; F:nuclear thyroid hormone receptor binding; ISO:MGI.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:MGI.
DR GO; GO:0003712; F:transcription coregulator activity; ISO:MGI.
DR GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IC:ComplexPortal.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; ISO:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IC:ComplexPortal.
DR GO; GO:0006366; P:transcription by RNA polymerase II; ISO:MGI.
DR InterPro; IPR019258; Mediator_Med4.
DR PANTHER; PTHR13208; PTHR13208; 1.
DR Pfam; PF10018; Med4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Activator; Alternative splicing; Coiled coil;
KW Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9NPJ6"
FT CHAIN 2..270
FT /note="Mediator of RNA polymerase II transcription subunit
FT 4"
FT /id="PRO_0000096384"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 227..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 26..131
FT /evidence="ECO:0000255"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPJ6"
FT MOD_RES 32
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NPJ6"
FT VAR_SEQ 171..177
FT /note="DPRRPYP -> NRASYIR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027918"
FT VAR_SEQ 178..270
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027919"
SQ SEQUENCE 270 AA; 29781 MW; A827D43766AAAD52 CRC64;
MAASSSGEKE KERMGGVSGM AGLGSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQLE
EENQVLELLI HRDGDFQELM KLALNQGKVH HEMQALEKEV EKRDSDIQQL QKQLKEAEQI
LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL
EMRSGLLGQM NNPSTSGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSVN MLPPNHSSDF
LLEPPGHNKE NEDDVEVMST DSSSSSSDSD
