ID   MED4_PICST              Reviewed;         302 AA.
AC   A3LYF7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
DE   AltName: Full=Mediator complex subunit 4;
GN   Name=MED4; ORFNames=PICST_62182;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000500; ABN67972.2; -; Genomic_DNA.
DR   RefSeq; XP_001386001.2; XM_001385964.1.
DR   AlphaFoldDB; A3LYF7; -.
DR   SMR; A3LYF7; -.
DR   STRING; 4924.XP_001386001.2; -.
DR   EnsemblFungi; ABN67972; ABN67972; PICST_62182.
DR   GeneID; 4840161; -.
DR   KEGG; pic:PICST_62182; -.
DR   eggNOG; ENOG502RXM0; Eukaryota.
DR   HOGENOM; CLU_071875_0_0_1; -.
DR   InParanoid; A3LYF7; -.
DR   OMA; MKLAKFT; -.
DR   OrthoDB; 1527310at2759; -.
DR   Proteomes; UP000002258; Chromosome 6.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..302
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   4"
FT                   /id="PRO_0000302080"
FT   REGION          239..302
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          98..145
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        239..265
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   302 AA;  33545 MW;  649DA6ADD016925D CRC64;
     MFPHKKNDPL KSLPISRVGS SQRLNQHISS IPSTPNYVAS SLNPVRNLPV SSGSIKSKIT
     TKAALDKFEQ LPIVKQVGAF EDTLNAISDN ISQFKEGQLH ANVEKIIAIN DDLKSKIEEL
     DRHRRLGENI KELEAESSNL DNTSKYILKE LISYRNELRA LPRLPASASK GSGDTVEIED
     ILKYAMKLAK FTKAPATSAS MPFQIHPNNY VWPAEDALRR GMLAASSLNP DEIIANELGT
     TEEEKIQEKK DEQVKKADKQ QDTGIRRGSF GDYGSSSSGK KKEEQSAQLD LDLFDSEDEF
     SD