ID   MED4_RAT                Reviewed;         270 AA.
AC   Q561Q8;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
DE   AltName: Full=Mediator complex subunit 4;
GN   Name=Med4;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Thymus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC       MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC       MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC       MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC       CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC       module termed the CDK8 module. Mediator containing the CDK8 module is
CC       less active than Mediator lacking this module in supporting
CC       transcriptional activation. Individual preparations of the Mediator
CC       complex lacking one or more distinct subunits have been variously
CC       termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
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DR   EMBL; BC093402; AAH93402.1; -; mRNA.
DR   RefSeq; NP_001019427.1; NM_001024256.1.
DR   AlphaFoldDB; Q561Q8; -.
DR   SMR; Q561Q8; -.
DR   STRING; 10116.ENSRNOP00000023195; -.
DR   jPOST; Q561Q8; -.
DR   PaxDb; Q561Q8; -.
DR   PRIDE; Q561Q8; -.
DR   GeneID; 306030; -.
DR   KEGG; rno:306030; -.
DR   UCSC; RGD:1306671; rat.
DR   CTD; 29079; -.
DR   RGD; 1306671; Med4.
DR   VEuPathDB; HostDB:ENSRNOG00000017170; -.
DR   eggNOG; KOG4552; Eukaryota.
DR   HOGENOM; CLU_082233_0_0_1; -.
DR   InParanoid; Q561Q8; -.
DR   OMA; NDEVEVM; -.
DR   OrthoDB; 1333917at2759; -.
DR   PhylomeDB; Q561Q8; -.
DR   TreeFam; TF324421; -.
DR   PRO; PR:Q561Q8; -.
DR   Proteomes; UP000002494; Chromosome 15.
DR   Bgee; ENSRNOG00000017170; Expressed in skeletal muscle tissue and 20 other tissues.
DR   Genevisible; Q561Q8; RN.
DR   GO; GO:0070847; C:core mediator complex; ISO:RGD.
DR   GO; GO:0016592; C:mediator complex; ISO:RGD.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0046966; F:nuclear thyroid hormone receptor binding; ISO:RGD.
DR   GO; GO:0003713; F:transcription coactivator activity; ISO:RGD.
DR   GO; GO:0003712; F:transcription coregulator activity; ISO:RGD.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; ISO:RGD.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; ISO:RGD.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Activator; Coiled coil; Nucleus; Phosphoprotein;
KW   Reference proteome; Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NPJ6"
FT   CHAIN           2..270
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   4"
FT                   /id="PRO_0000302064"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          227..270
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          26..131
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..15
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NPJ6"
FT   MOD_RES         32
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NPJ6"
SQ   SEQUENCE   270 AA;  29826 MW;  AC73902766A3DD52 CRC64;
     MAASSSGEKE KERMGGVSGM TGLGSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQLE
     EENQVLELLI HRDGDFQELM KLALNQGKVH HEMQALEKEV EKRDSDIQQL QKQLKEAEQI
     LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL
     EMRSGLLGQM NNPSTSGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSVN MLPPNHSTDF
     LLEPPGHNKE NEDDVEVMST DSSSSSSDSD