ID   MED4_YARLI              Reviewed;         336 AA.
AC   Q8WZL6; Q6C3B1;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 4;
DE   AltName: Full=Mediator complex subunit 4;
DE   AltName: Full=YlMED4;
GN   Name=MED4; OrderedLocusNames=YALI0F01144g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SY12;
RX   PubMed=11861549; DOI=10.1093/genetics/160.2.417;
RA   Gonzalez-Lopez C.I., Szabo R., Blanchin-Roland S., Gaillardin C.;
RT   "Genetic control of extracellular protease synthesis in the yeast Yarrowia
RT   lipolytica.";
RL   Genetics 160:417-427(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 4 family.
CC       {ECO:0000305}.
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DR   EMBL; AJ315749; CAC86052.1; -; Genomic_DNA.
DR   EMBL; CR382132; CAG77653.1; -; Genomic_DNA.
DR   RefSeq; XP_504851.1; XM_504851.1.
DR   AlphaFoldDB; Q8WZL6; -.
DR   SMR; Q8WZL6; -.
DR   STRING; 4952.CAG77653; -.
DR   EnsemblFungi; CAG77653; CAG77653; YALI0_F01144g.
DR   GeneID; 2907721; -.
DR   KEGG; yli:YALI0F01144g; -.
DR   VEuPathDB; FungiDB:YALI0_F01144g; -.
DR   HOGENOM; CLU_826926_0_0_1; -.
DR   InParanoid; Q8WZL6; -.
DR   OMA; MKLAKFT; -.
DR   Proteomes; UP000001300; Chromosome F.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019258; Mediator_Med4.
DR   PANTHER; PTHR13208; PTHR13208; 1.
DR   Pfam; PF10018; Med4; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..336
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   4"
FT                   /id="PRO_0000302081"
FT   REGION          1..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          59..85
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          281..336
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          154..192
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   336 AA;  37035 MW;  12749A18E72B528F CRC64;
     MNSTPANSTP LPPLQPFTPG SSAATPAPIA LTRSKPRRRL SSNVVGVSDR RRLLQMLQNV
     EEVSPGPDSA SEAGESPSIR SQDEAHLEHL SSARICMDED KELLIHQALP AFENTLLRFV
     QALSKYDFRQ DLAETLMETE SQFCEAVDEL VEHQQAAQTI AALERVSEGL DDKIRDMIRK
     LAECRRELRN YQPNNENQST LSSADLLTYA TRIANFTTAP PYFRERPEHS KLPWPIEDEM
     RKGLLALMEV GKDKTELGEL ADPEKFAHTA ANGVAPNGAA PVANGVAQNH NNGYAMERRL
     STGYGSDNDG DTNMNGRSGL AGLDIFDDDD DDDDDD