ID   MED5_CANGA              Reviewed;        1099 AA.
AC   Q6FV36;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 5;
DE   AltName: Full=Mediator complex subunit 5;
GN   Name=NUT1; Synonyms=MED5; OrderedLocusNames=CAGL0E05060g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 5 family.
CC       {ECO:0000305}.
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DR   EMBL; CR380951; CAG58827.1; -; Genomic_DNA.
DR   RefSeq; XP_445908.1; XM_445908.1.
DR   AlphaFoldDB; Q6FV36; -.
DR   STRING; 5478.XP_445908.1; -.
DR   PRIDE; Q6FV36; -.
DR   EnsemblFungi; CAG58827; CAG58827; CAGL0E05060g.
DR   GeneID; 2887379; -.
DR   KEGG; cgr:CAGL0E05060g; -.
DR   CGD; CAL0129110; NUT1.
DR   VEuPathDB; FungiDB:CAGL0E05060g; -.
DR   eggNOG; ENOG502R1HB; Eukaryota.
DR   HOGENOM; CLU_281615_0_0_1; -.
DR   InParanoid; Q6FV36; -.
DR   OMA; FTCFAQF; -.
DR   Proteomes; UP000002428; Chromosome E.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0043966; P:histone H3 acetylation; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR014801; Mediator_Med5_fun.
DR   PANTHER; PTHR35784; PTHR35784; 1.
DR   Pfam; PF08689; Med5; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1099
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   5"
FT                   /id="PRO_0000302771"
FT   REGION          41..66
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        48..65
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1099 AA;  125498 MW;  92A4F440AB4ED6BC CRC64;
     MESKSLNTLA LRCAARKIPA SEFLNLYKEF YNETFPANSI DNDDAKTQEG SGSQDKTDVE
     ESISKPVGSK NDPVAILCAE FMKLLENGKY VILADYVVEV LFVNYHSELV REFLPKLKDL
     MNKGILIHFF SKSCAFFVNL TDNLVISQLI KDLRATIVPC ILETNFCDIS NELVVAIAKF
     LQAVLRFTPQ PIQINSETYR DNTFNLTKRL SLINKILSKK FAGIIDKKLQ FKEVLGPFTK
     DSTLDFDNSP SITSPQFIPS PLPSMKERSV TSSQSAIKYK DLKLLRYYKN IWLNSKLMNW
     QPFDSEFISN YSAIKSSLYP DQVQNIQNVD LLFTDLIETA FTCFAQFVSN KLYHQSNSTY
     NLLERKWILF LSKILPLLVY KNSSRTAHVI GNALDGIDDK VIKAISAYYQ ENDDGRSRND
     DLFDDYPSTS LDIRHDFIKS LTMLGVVPPV FITNYLRGDQ TVDSKALATT DDLTFTNQQG
     IIEIVNDIPN FIRSSLEGME MENVSEPTLV SSNGLLQVLS NFDTVSPTKQ FELANAIVDM
     LSESSTTFEL NTFVKLTAVL TFNYSHSLTS ILMYVTPEVL TKLYLEFVDK HWNSQVIGKQ
     ETDGESQFEN VNISMSFSWA ILMLTILYKQ YHIDFVSMRA DYTTDNIKNS FAITFVENLP
     DISDLFFIDE KNSDDPEVQV KSHKLVRDWL NDLFVNGSLS DSLLQNIEPK QLAILVPYIF
     KQVTLAMEIG AVGDLQNLIG GFEYFLQPFM LVGLIKVMYW LEQYLSCLKS DETDEKLIQK
     VLSLLNTIIC PSTLNEDSKA FHFAVLRLNA IPLLGILYQF RSNKHAESNY GIYSSDNEGN
     PTLELMISRL ISSLSISPVY DIDSTILVTD NNFVQKPPKF QSFFVTNEIS MNKMLTNQMN
     SFWSLHSSTY YNLDFLKTLI DTLTPKQFLL DVLRTLEYKV ETLGVKDVRN KSSSNESDQV
     IDYLFYFLVL YDIENSEDAK AMAQFMEDTV DISINGDSGI VKQETQPKSE YNPDDDIDML
     FGENDTSMQA NEEDTLDNKE LKSDRNCALG KNRHTFGFII HEIKLSYGTL ESDSMSYEDY
     KKICEYHSRY LKMLKTCIF