ID   MED5_COCIM              Reviewed;        1052 AA.
AC   Q1E024; J3KD71;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   03-AUG-2022, entry version 68.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 5;
DE   AltName: Full=Mediator complex subunit 5;
GN   Name=NUT1; Synonyms=MED5; ORFNames=CIMG_04089;
OS   Coccidioides immitis (strain RS) (Valley fever fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX   NCBI_TaxID=246410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RS;
RX   PubMed=19717792; DOI=10.1101/gr.087551.108;
RA   Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA   Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA   McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA   Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA   Henn M.R., Birren B.W., Taylor J.W.;
RT   "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT   and their relatives.";
RL   Genome Res. 19:1722-1731(2009).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=RS;
RX   PubMed=20516208; DOI=10.1101/gr.103911.109;
RA   Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J.,
RA   Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D.,
RA   Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A.,
RA   FitzGerald M., Lui A., Macdonald J.P., Priest M., Orbach M.J.,
RA   Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R.,
RA   Taylor J.W., Rounsley S.D.;
RT   "Population genomic sequencing of Coccidioides fungi reveals recent
RT   hybridization and transposon control.";
RL   Genome Res. 20:938-946(2010).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 5 family.
CC       {ECO:0000305}.
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DR   EMBL; GG704916; EAS33065.3; -; Genomic_DNA.
DR   RefSeq; XP_001244648.2; XM_001244647.2.
DR   AlphaFoldDB; Q1E024; -.
DR   STRING; 246410.Q1E024; -.
DR   PRIDE; Q1E024; -.
DR   EnsemblFungi; EAS33065; EAS33065; CIMG_04089.
DR   GeneID; 4562523; -.
DR   KEGG; cim:CIMG_04089; -.
DR   VEuPathDB; FungiDB:CIMG_04089; -.
DR   InParanoid; Q1E024; -.
DR   OMA; CNQKETM; -.
DR   OrthoDB; 1352388at2759; -.
DR   Proteomes; UP000001261; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR014801; Mediator_Med5_fun.
DR   PANTHER; PTHR35784; PTHR35784; 1.
DR   Pfam; PF08689; Med5; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1052
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   5"
FT                   /id="PRO_0000302772"
FT   REGION          949..982
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        961..977
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1052 AA;  115052 MW;  361A38A584293BD8 CRC64;
     MAYLTDDKAS GPEWALLLEQ CISHRIISTE FRDFSVIMMR RHPISEKALI RIVLEARSAT
     GLMWDPLIPL YVEVLQKLGY VSLPEILKTL LLFSTIYDDS HPVSKAGPNG KGVKRKKKTS
     TLMTDNKIIQ NVMATITMGQ GPKATRGATD VLCAVADWIS TLLAWSPSGE GPESDQPGDI
     LGHQDGACIF GSLGLLLVAL VGTEKGVSAL SSLSKKDRKC LGQALSSYTP ICASYSLPLR
     NRLDSIQKDF NIYPSDSSKG LEESMMGDVN VAVLQFESNV VDIPTTSSRA GLYIYINALL
     AGRPLIDDSM FLNYLNNRYG GDHISMVEEL ITAAFDVLSN GMYRNESSKT MFLFRAFLVN
     KLPPFLAYMS ASSMSQIPME VCITRALSRV DPNTFPSFSE TFSMQGNSVL SDVRQEFLFS
     CALHKLIPES SIERLLGENP MQTLPVGGQF MKDTLIAQIN NNPERAEQLL NGIESMEGNA
     GAIVDAIIEV MHNLCSRKET MTLKSICNSL SRRPQTLDII LLFKSPSFIL QPLCSLLDAW
     KWDEDQGESQ LVYDEFGSIL LLVLAFKYKY DLTPLDLGIN KPDSFILRLL DTGASSQQLK
     DLTEKQNQNL GAWITALFVA EGISDESMSS CSPQEFYLLV ATLFSQSLTA CEAGKMEFET
     LKGGFEYLLE PFLLPALVMA LTWLGNHIWE SESDLETSLK ILHGLVKPAS ISGEAQEIHR
     TVLCIASRTL EATLKDTRAR HPSRTDISPI LDVLEQYHSF RRTGATNQTE LDSWRANPAG
     GGLVTSIRNT FSSLVLWSTD PEISMTPPSY THRQMLAGIG HVGSERVLQG LIDELKLQTE
     TGSGNLAFDI AATLICAPMA ESFTLEQILY RQMDQDKYPL PGCRMLTLRD ALGIQRESLS
     KLIEADPHRA ELIIRLHRRV EALCSIPQIA PGDVDVGNIM DSIHLEAVGG DDEQREQHQQ
     QQPDADQSNQ GVVAPTGNTP GNLDAMLDAA ASAVVADPNG TAGAEAAAGA GLLGNGVGGD
     AGVGIGTGMN DVFSLMDMGN PEFIDLDMED ML