ID   MED5_DEBHA              Reviewed;         990 AA.
AC   Q6BH05;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 2.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 5;
DE   AltName: Full=Mediator complex subunit 5;
GN   Name=NUT1; Synonyms=MED5; OrderedLocusNames=DEHA2G22352g;
OS   Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990
OS   / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX   NCBI_TaxID=284592;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 5 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382139; CAG91026.2; -; Genomic_DNA.
DR   RefSeq; XP_462516.2; XM_462516.1.
DR   AlphaFoldDB; Q6BH05; -.
DR   STRING; 4959.XP_462516.2; -.
DR   EnsemblFungi; CAG91026; CAG91026; DEHA2G22352g.
DR   GeneID; 2905468; -.
DR   KEGG; dha:DEHA2G22352g; -.
DR   VEuPathDB; FungiDB:DEHA2G22352g; -.
DR   eggNOG; ENOG502R1HB; Eukaryota.
DR   HOGENOM; CLU_012200_0_0_1; -.
DR   InParanoid; Q6BH05; -.
DR   OMA; MVINICG; -.
DR   OrthoDB; 1352388at2759; -.
DR   Proteomes; UP000000599; Chromosome G.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR014801; Mediator_Med5_fun.
DR   PANTHER; PTHR35784; PTHR35784; 1.
DR   Pfam; PF08689; Med5; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..990
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   5"
FT                   /id="PRO_0000302773"
SQ   SEQUENCE   990 AA;  113960 MW;  14BF5301386C035E CRC64;
     MASSTATVSL HNLVKLCCKR NSTSKVFVSL YNQLEEKSPI QDNDYLSNLL QPDIKNKSKR
     ESSLITEYIL ALAISSESQL EKFWKFLPEI LLENQIHYMI ELNSILLSKS NIERPSSDIF
     KEIVNNCFFK YTSSYISSLF PGISATQVSL LNHIIVVWCS VIRRSPFHLN TSSFKQLAVK
     IVSVLTECDL RNSLNYFISN TSSILNTDDL QVDSVQDSDD KKIAAIRSTT TIQLVNNISS
     INPKSRKATQ LNVIKRYLWL NSIMKNWKFS NDVFLKQFEQ NFLPNSSNNL KKPYVLAFEL
     ICAFFKGIVT SICSNESKYV LFNWKNFIIT RLPTLLNDIK FTTSHVAPTS NDSSFKQETL
     DDAIINAFES FEDPVNKVLT QFSIDNLSIC DLRQTFIKSC IYNKLIPVMS YHKIFTADNT
     VTDQFLNNGI EQVGQITSIR DEFETKLLNI NTEFTTLEDS GLIEYINSLP GLLEFSATKQ
     LELSETVDQV IEHLIDEKNI EKLYRLLLSV CNNLTTLNLI CFNSNCGPFS ILYKLINYID
     NEDFNVDDDD NFQETYAFFG IIILAILLII ETFKIDYSQI TVTNSYIVDY INEFYYRLCD
     SLTNISTSDT EEDNTIISNY NDLISDWINA LFDDNNDGLS DDLIKSVNVK QIYKMIPIIY
     QQSIIATNSN KIDIKILNNG IDYLSQMFLI PCTLNIIKWL LRRIWADDPT LDCLPVQVLH
     ELIKSNMGES DEPTSESKLF FQIVLKISGN SIISTLKKLN NWENSNLIKD IIEKVSNSVD
     PMYLENDLSI KLNERVNLYE QLKTNVINLV NNMNIYDYQF LNIFSSIDRD NELIRYIVEE
     ITSYQKTNGG NEDTKLFINL SIFMILLDAI DNDDDKQYWI KRIKDTMVPV SSNSSGENRF
     DISMDYHYSS IFNNSDETSN NDYLFSGENN NSSVEIEGLK RKIGRHESLL NSFNKIKEFC
     DTEVQSTFVK SLRTFRDKLI NELEVFTLNV