ID   MED5_EMENI              Reviewed;        1054 AA.
AC   Q5B503; C8V8W6;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 5;
DE   AltName: Full=Mediator complex subunit 5;
GN   Name=nut1; Synonyms=med5; ORFNames=AN4377;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 5 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CBF77651.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=EAA60294.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AACD01000076; EAA60294.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; BN001303; CBF77651.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_661981.1; XM_656889.1.
DR   AlphaFoldDB; Q5B503; -.
DR   STRING; 162425.CADANIAP00006081; -.
DR   EnsemblFungi; EAA60294; EAA60294; AN4377.2.
DR   GeneID; 2872175; -.
DR   KEGG; ani:AN4377.2; -.
DR   eggNOG; ENOG502R1HB; Eukaryota.
DR   HOGENOM; CLU_004096_0_0_1; -.
DR   InParanoid; Q5B503; -.
DR   OrthoDB; 1352388at2759; -.
DR   Proteomes; UP000000560; Chromosome III.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR014801; Mediator_Med5_fun.
DR   PANTHER; PTHR35784; PTHR35784; 1.
DR   Pfam; PF08689; Med5; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1054
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   5"
FT                   /id="PRO_0000302780"
SQ   SEQUENCE   1054 AA;  116600 MW;  2A728925BCD186B7 CRC64;
     MASEQWRAFV QRCLLHRIDA AEFRDLSMLL FQRCPIGDLL LTETLLQARL ATGIKWDPLL
     PVYIDCLCRM GRVQIPTVLT ALLKHSSIHD KPSPGADVAS VKKNAKPRAK CYTLMTDIRV
     IQDAMLSINS GTTVKTLLEV IHTFCAIADW IQAIIAWHNS QLDADQPAGM MGSPDAMLLF
     EAVGILLSVL AGTGKGIEVL SAGPHEATKI RIGQALSAYL PLCADMYPPL HNRLDGLQKQ
     FNLYGQPPSK LDVTMMDNVN VNALQFEASV MDGPTIHSRA GLYIYINAML VGRPLVDDSI
     LLNYLYNRYA EHYDVLIEEI ITASFDVLSN AMYRNESART MLLFRSFLVN KLPPFFAAML
     AVPMVTFPME ECISHALSRI DPNIFPSFSQ MFSMQGNAAL SDVRQEFLFS CASHKLIPES
     SIERLLGENP MQTLPVGGSY MKDELKAQIS ANHERAEQLI SDIESTEGNA GAIVGAITEV
     MHDLCMQKET MTLKNICNSL SRRPQSLDVM LLFRSPRQIL QPLCALLDSW HWDEDQGESQ
     PVYDEFGSIL LLVLTFKYRY DLRPWDLGIA SNDSFIIKLI EQGSSSQKLD ELTEKQNKNL
     GEWIAALFIA EGISEETMSA CSPQEFYMLV STLFSQSLGA CEAGKLEFDT LKGGFEYLLE
     PFLLPSLVVA LTWLGNHIWE SEADPTIPLR TLQSLVSPSS ISGEAREIHL TVLNITSRSL
     EEQLKDVRAR HPTRTDIKPI LDALDPCLSF QRTASCHKSE LEPWTTHNPG GLLGSIRATF
     QSLILWSTSP DVSMAPPSYT HRQVITAIRT LGASRVLAAL LDELKFQTES NPGTAGIALD
     IAASIVSAPL SESFAFDQFQ THHDHSTSKE SEQAPHYPIF NLRDALNTQH ELVPKLSEKD
     LLRAEIIVRL YRRVNTLLAP PAQVAPNLDM NMGMNVNVNM NMNNIIGDMH LDSSGDGASN
     QNMNMNMETD TRTQAQEQGH NNEQANLNQI MDNAAAAVGM DMDMNMGGGG DGMGVGVGVG
     ELGLGEVDQS IDDVLNAAEM NPEFLDLDME GMFQ