ID   MED5_KLULA              Reviewed;        1067 AA.
AC   Q6CNY5;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 5;
DE   AltName: Full=Mediator complex subunit 5;
GN   Name=NUT1; Synonyms=MED5; OrderedLocusNames=KLLA0E08943g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 5 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382125; CAG99441.1; -; Genomic_DNA.
DR   RefSeq; XP_454354.1; XM_454354.1.
DR   AlphaFoldDB; Q6CNY5; -.
DR   STRING; 28985.XP_454354.1; -.
DR   EnsemblFungi; CAG99441; CAG99441; KLLA0_E08977g.
DR   GeneID; 2893802; -.
DR   KEGG; kla:KLLA0_E08977g; -.
DR   eggNOG; ENOG502R1HB; Eukaryota.
DR   HOGENOM; CLU_281615_0_0_1; -.
DR   InParanoid; Q6CNY5; -.
DR   OMA; FTCFAQF; -.
DR   Proteomes; UP000000598; Chromosome E.
DR   GO; GO:0070847; C:core mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0043966; P:histone H3 acetylation; IEA:EnsemblFungi.
DR   GO; GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IEA:EnsemblFungi.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IEA:EnsemblFungi.
DR   InterPro; IPR014801; Mediator_Med5_fun.
DR   PANTHER; PTHR35784; PTHR35784; 1.
DR   Pfam; PF08689; Med5; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1067
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   5"
FT                   /id="PRO_0000302781"
SQ   SEQUENCE   1067 AA;  121456 MW;  84508FEA8AC044A2 CRC64;
     MESVSQLVSN CASRGIPAQQ FLNFYHELIN EKYGASLNAG NEERESESRK QVFEDVSREL
     VQAFHSSEDA ILLAEYMVHL LFINYDTYLS SALLPQVYSI QSETLLMHFH SLAASFIGKL
     EDKLIKEQMR QDLSTFILSS CLQCDMSTFN NQLFISIVKY LQALLTLIDD SIDIEMDKDN
     LKLAVTSLLT RTTKLNRILG KRIGREFETK LNLSLSAKLA MVNSPQIFSP SSGATRFAGT
     PGSTKPMDLA LSATSSTSNK IQDLKLIRFY KNLWLNNKIH HFNTSDSQLL EKFSSINSRL
     SSSLAEEHLI QEQITDLIET TYTSFAQLVN NKLYHQTNTN FNLLERKYVH FITKRLPLII
     KDFLPQNPSV IVNSLQNIDE KVKKAIKSYY SNKNDSPERN EDLFDDYSGN NFDIRHDFLK
     NLIMLNLRPP SVLNEFLRED QMVDVKTLKT DDSLVIVNSQ GVQEKVSDVQ EALKSLIADL
     DIENQYVANG NNYRFSPDNA LLKLLMSFDT IAPTKQVEIA NAFLQILQKA TDTSDLKTLG
     KVLWILTSNV GHSTTSMLCC ISPGPFINAV VKFLEKQQRN STVSSSDEPD FDSLHSYVTY
     GMALSFVVFL KTTYDFDIEP FIQNFEESYT LKFLSSIEDI SDIFVLQNES AEGSKLYLQN
     WLRDLFINGS ISDSQMKNTN VKDLINLIPF IFKQSVIAVQ AGVVSNILNL TSGFEYFLQP
     FLMPGLIKIM FWLEYYLHSL KNNNPPSQIL SSCWELINIL ICPPSLDDDA KGLHFMILKL
     NCVRLLNVLY LFRNDESNSS QYGVYAAHES IDPKLEAVIS KLEYIASISN IYDVDTKCYE
     TTKEVYSHGT LFSNKIPVTN EHPIDIIMTN QLNSFWNLHS STYYNYDYLL ELIKLITPEK
     FLVDSIRTLT YKVAAYGIPG VQGKLNTSAI EQVANFLAYF MILHDVQTEE QRLALLNYIE
     SGVIVSKETE TKQEPETKLD DDFDMLFGEP FNNTLDETSL VFSQNEQPKS YSSSVFPVLH
     DTFGVVIAEM KKRYDTSKNN GLLSEAAYEN ATTFIRKYVE NLKNSVV