ID   MED5_NEOFI              Reviewed;        1009 AA.
AC   A1CXW3;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 5;
DE   AltName: Full=Mediator complex subunit 5;
GN   Name=nut1; Synonyms=med5; ORFNames=NFIA_109590;
OS   Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164
OS   / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=331117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181
RC   / WB 181;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 5 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAW25465.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; DS027685; EAW25465.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_001267362.1; XM_001267361.1.
DR   AlphaFoldDB; A1CXW3; -.
DR   STRING; 36630.CADNFIAP00009838; -.
DR   EnsemblFungi; EAW25465; EAW25465; NFIA_109590.
DR   GeneID; 4593457; -.
DR   KEGG; nfi:NFIA_109590; -.
DR   eggNOG; ENOG502R1HB; Eukaryota.
DR   OrthoDB; 1352388at2759; -.
DR   Proteomes; UP000006702; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR014801; Mediator_Med5_fun.
DR   PANTHER; PTHR35784; PTHR35784; 1.
DR   Pfam; PF08689; Med5; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1009
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   5"
FT                   /id="PRO_0000302782"
SQ   SEQUENCE   1009 AA;  111160 MW;  0705D4296AE2A57F CRC64;
     MTAPVQWRTF FHQCLIHRID ASEFRNLSKL LFQKCPIAES ALLDALLQTR SESRIKWDPL
     LPLYIDCLCR MGRVRTSTVL TSLLKYSSIH DKPQLPTSEG KDGSKCYTLM TDIRVIQDAM
     LSVSTGSTPK TNAEAIAIFF AIIDWIHAVA SWHNSHFDPG QHSSGMMSSP DVVSLFESLG
     ILLAALSGTG KGLEVLSADS HEGLKVKLGQ ALSAYLPLCV EVSLPLRNRL DGLQKEFNLY
     GERAPKSLDV PMMENMNVNA LQFEASVMDG PVINSRAGLY VFINAMLVGR PLVDDSMLIN
     YLANRYGGHY EALIEEILTA AFDVLSNGMY RNESSRTMFV FRSFLVNKLP AFFAAMLAST
     MVSIPMEMCI SHALSRLDPN TFPSFSQMFE MQGNTVLSDV RQEFLFACAS HKLIPESSIE
     RLLGENPMQT LPVGYNKDEL VSQINANPER AEQLINEIES MEGNAGAIIG AITEVMHNLC
     NQKETMTLKN ICNSLSRRPQ ALDVVLIFRN PKQVLQPLCA LLDSWHWDED QGEYQPVYDE
     FGSILLLVLA FKYRFDLRPA DLGISSNDSF VLRLLERGTC SQKLDALDEK QNKNLGSWIA
     ALFIAEGISE ETMSACSPQE FYLLVATLFS QSLEACETGK LEFDTLKGGF EYLLEPFLLP
     SLIFALTWLG DHIWETELDP SIPLKALQSL VNPSSISGEA REIHRTVLNI TARTLEEQLK
     DVRTRHPSRT DIKPILDSLE PCLSFQLVGS SHRSELESWT THSGGGLLGG IRSTFQSLVL
     WSTNPEVSMA PPPYTHRQLI AGVRMLGASR VLPPLIEELK LQTEAGNGPL ALDLAATLIC
     APMAETFSVE QNSHQPVDPN KEALPRCGIL TLRDVLALQH ENVPKISEKD PLRAEVLVRL
     YRRVNALLAP PSQVPNLDVN NIIQNMQLGG VGVGVGAGQM ELDAAGAADH GVGPDDAENI
     HRMIDNAAAA AALDSSGTGL DTSIDDVLNA ADMAVGNPEF LDLDMEGMF