ID   MED5_PICST              Reviewed;         981 AA.
AC   A3M022;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 5;
DE   AltName: Full=Mediator complex subunit 5;
GN   Name=NUT1; Synonyms=MED5; ORFNames=PICST_64197;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 5 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000502; ABN68443.2; -; Genomic_DNA.
DR   RefSeq; XP_001386472.2; XM_001386435.1.
DR   AlphaFoldDB; A3M022; -.
DR   SMR; A3M022; -.
DR   STRING; 4924.XP_001386472.2; -.
DR   EnsemblFungi; ABN68443; ABN68443; PICST_64197.
DR   GeneID; 4840875; -.
DR   KEGG; pic:PICST_64197; -.
DR   eggNOG; ENOG502R1HB; Eukaryota.
DR   HOGENOM; CLU_012200_0_0_1; -.
DR   InParanoid; A3M022; -.
DR   OMA; MVINICG; -.
DR   OrthoDB; 1352388at2759; -.
DR   Proteomes; UP000002258; Chromosome 8.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR014801; Mediator_Med5_fun.
DR   PANTHER; PTHR35784; PTHR35784; 1.
DR   Pfam; PF08689; Med5; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..981
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   5"
FT                   /id="PRO_0000302784"
SQ   SEQUENCE   981 AA;  111847 MW;  87FBCFABE8FAB46B CRC64;
     MSETVSLHKL VKKAVHSKLS FKTFLGLFSQ LNTKQVVQDG EYQTEILEIV QNPRNLNDKK
     ITEYKVVLAI QLSLSSSEEL KRFWGNLSKI SLVGQVDYLI RLNKILKYEY SKYDKDIVRL
     LIKVQYCDYI VEVLDSFGDK KLSTEQKSLA NHIVFFASSV IEHVVVKDEA LVNDLVFQLA
     TRLEGLRLSN LLDFLLLKSK NILSEDQIRL TDSSGSGEDK SSDLPTITTS LYKNLSVGSL
     SSAKQETYNE TIKFLWLNKI MKSWKFADNE SIFNNFVHNF IPSSDKNKNP YLTSFELIKA
     TFKGFGYAVI NNDDCYVLFN WKNFIITRIP VILSTLKFAN VNDEETLERA VLNAFNSLPD
     SIVKVLTNLA SGSTKVYDLR QIFIKSLIFN RLLPPVAFQK FFPMESKITQ QVILSELAQY
     NHDLNLRRKF NDKLINVNSE FTSLEESGLL ELCNSLPASL EYSYSRQIEL SNAINDIIDE
     MKISREHEKL NRLLLSVMSN VQLTNILVFN SNPYVVLGKM IDYIDSENFR IDDDDENFQD
     VYSYCGVLIL SIISIIEKFK IDLSTFNIKN SFALDYINNF YYRLCDDLTN QIPVNSDEED
     NTIATNYNNL LIDWINALFD DSNDGLSDDL IKSISIKQIY KLIPLVYQQA ITATSIGKID
     FSILTNGIDY LSQVFLIPTT VSIINWLLSE ISNKKTDAES LPVKVLSEIV KSNISSESGS
     QELSSLVFKI VVNICGSNIL IALKKIKDWE NSQRIRDVVS IVTANLDSNY VEKDLSLPES
     KSDININEQI KSHLVNFQQQ ADSPTPIHAF IHRFISDSKD QVLRVLLQEV TSYQKQNNEA
     TKIFINLASY LVVSSSIDSV EDKKYWHGYL SNVSSSATEH KILKNSDKEF NSSMDYHYSS
     IFNDASSGAS NDDDLFNDKS SKQLSSGKLL EQLRTKVNRY NNLLAKFNVI FAENQDPSSP
     WHKTVNTLAD KLLDDINDLY I