ID   MED5_USTMA              Reviewed;        1237 AA.
AC   Q4PAB8; A0A0D1E345;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 5;
DE   AltName: Full=Mediator complex subunit 5;
GN   Name=NUT1; Synonyms=MED5; ORFNames=UMAG_02945;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=521 / FGSC 9021;
RA   Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 5 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CM003146; KIS68965.1; -; Genomic_DNA.
DR   RefSeq; XP_011389352.1; XM_011391050.1.
DR   AlphaFoldDB; Q4PAB8; -.
DR   STRING; 5270.UM02945P0; -.
DR   EnsemblFungi; KIS68965; KIS68965; UMAG_02945.
DR   GeneID; 23563562; -.
DR   KEGG; uma:UMAG_02945; -.
DR   VEuPathDB; FungiDB:UMAG_02945; -.
DR   eggNOG; ENOG502S7J1; Eukaryota.
DR   HOGENOM; CLU_271915_0_0_1; -.
DR   InParanoid; Q4PAB8; -.
DR   OMA; ARWCKAL; -.
DR   OrthoDB; 234079at2759; -.
DR   Proteomes; UP000000561; Chromosome 7.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR014801; Mediator_Med5_fun.
DR   PANTHER; PTHR35784; PTHR35784; 1.
DR   Pfam; PF08689; Med5; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1237
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   5"
FT                   /id="PRO_0000302785"
FT   REGION          1109..1131
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1202..1226
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1237 AA;  132756 MW;  1A0F218DAE2E3BA5 CRC64;
     MSTGEAQQLI QKLLLEALAR RLAQPKWLSL LHQLVRRIEA AQDDDGTFDV IPSLEGSHAA
     ECLVKYLLSQ SFIDPLLIEY LQALIYGSAN RSGVSPDQGP ITDVMTVTLH LLANVQAAAS
     NVPAIEIISS VIGQGLLMTF QAPVPFRVSS PSFLALLQRL FADPTYEQDQ TPSFPVDHVD
     TGASDIKVAP ASLGFVVANL RLLALAADPA IASPQNLLAP RVSITVLINV GQGLLAYVTQ
     ILSAAQTAKN DKLKPDGAIL QQLRSMASTS LTEIEAVLRS MNPAWKDEIA LLRSLTSQIG
     VIDQISESFA EANTSKLSTR PTKRKLETLQ DAQQRATWDH FLDVSTIAAS EKPAVEPETA
     LLMHLLVDHH TSWDFKLDAI KTLFLARRSA AAPSLTLEKS LTAFYFELLV AAIDACAAVV
     EMPPAFKGAE VYATIWRNAL CGMIPELVLQ LEQWLDGNQE LPLRGQRLEA PHVRLEAALR
     AALLVMSDRL NVCESATNQS HAHGNVAATN GEEAAGAHAN MMSDVLGLGT PPSQPIRAWL
     LRACIEHSLA RPEAIADEFA NGHKLASEVQ SLTQSLRMDA QLEGLALNTL FETRIPTDHP
     VELLQRVASD PGTHFIFARQ LVLQVQGWLE QHDLESIARW CKALTENACE GGAMLDTIMI
     YIDPAQMLDP LASILDHQDL GQTSDEPSTL SDILLFVQLL CYRYSTPITR ISRYTVSNHD
     MDTVDSSAPL HRSMPPFLAT HLATSSVSYP LSVLSEEDRG LVSRWIHALF GNEGISDDLI
     SASPPTTLLR LSPLLFSQSI SACQHGIIDL ETLRGGLSYF LQDLLSFALP GALVWLLSEI
     SRTPLQPILD FLSESGLQAS VVDVPSSDGT LANGLPRNAT SKSVCLEVLA LLVDTDACPS
     SVRQLIAKAF DAFVSTIEAD VTAAPQLAVG CETFNLASLK ARMEGAGVTA RLLSETGASW
     LQSVAAGHQD SRSKLFSMLT TLQPSVHAMD RLIGALKSGQ VGRNGRDQKG LAAWLCFIAP
     ASEPTSALRF VQRLELGDIE ESAIEGVVRI VGLVVGLIMA AEAQRATTDA VADDLLALAR
     AADTNARGTK ATVADTTAAA RPAEADLFDD EPCSPQPPHA AANATSHTSN AAPTVTSAAS
     LLASGELRTT SKQVLDMMAL KLVRFRSHML GEKNAKSTTI SAKWATLQSA IADHLAGGEA
     WHGAQQCRER TQNDAPVNGG KDADSGSGLS AWLSAMS