ID   MED5_YARLI              Reviewed;         991 AA.
AC   Q6C8D1;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 5;
DE   AltName: Full=Mediator complex subunit 5;
GN   Name=NUT1; Synonyms=MED5; OrderedLocusNames=YALI0D20636g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 5 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382130; CAG81273.2; -; Genomic_DNA.
DR   RefSeq; XP_503081.2; XM_503081.2.
DR   AlphaFoldDB; Q6C8D1; -.
DR   STRING; 4952.CAG81273; -.
DR   EnsemblFungi; CAG81273; CAG81273; YALI0_D20636g.
DR   GeneID; 2910334; -.
DR   KEGG; yli:YALI0D20636g; -.
DR   VEuPathDB; FungiDB:YALI0_D20636g; -.
DR   HOGENOM; CLU_301519_0_0_1; -.
DR   InParanoid; Q6C8D1; -.
DR   Proteomes; UP000001300; Chromosome D.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR014801; Mediator_Med5_fun.
DR   PANTHER; PTHR35784; PTHR35784; 1.
DR   Pfam; PF08689; Med5; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..991
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   5"
FT                   /id="PRO_0000302786"
SQ   SEQUENCE   991 AA;  109072 MW;  7FFAAAF5D60B65C5 CRC64;
     MDFNDKLATL TRVCLARRVS AGRFVKLTRH LMENEGASEV NPKALFGANT FDLLFFAYVH
     KLAFEDSLLP PYKLLAYLKV CAYGRLPQLL MYLGKQLQEM DTDVDLGANA GYSYLIQVLV
     SFMKDAFASG GGMRKRYGTS IAKGFGFFAE VLLTRLGDRM AGVTAPEITS QLAELAKVDA
     TIAGRLGEAW MGLKGAAKTS TTAETVAATV ATPTTLTRLY RDTLFESLMF GDSSWNQYGS
     LFQLISTVYA MPGYPTLASQ PVQSRLAIIG EVICAMFDCQ SITFLRRDSA KRNNYWKSCI
     RTRIPRIIRT LFPHSTPEEK TLIQSVVSKS VLGLDQPTVT LIRLSIGSDE LDDMFSSFPG
     GLDVDIRHEL VAACISEGVL PKDAFNAIFK DSGISVEALD YADELTIDGQ AMSLDTMAEQ
     LSYENLEIGS VSDCLMSKVV AQMEKLSGNA QEALTKRLLV CLADFVQAGN VRPVRYMCQA
     LALNTTAVDI MALCVDPMLL LKALVDCIEL WREEEDEANY QEAYTDFGAI VVFAITLKQR
     YHLDLALLNQ FGTLFVGAPS PSSTMPSHEL FLSQLLTESD SSQRIFHLSD EQNDQLSGWA
     TALYDAGGIS DDLMRSSSVK QFFLVTPAIF QQSMLAHEKG LLSLSTFKGG LEYFLQPFLL
     GSTVGVFKWL SEQLWVHSQK GHDEQMSTVI SVCSTLILTD NTDIEVLSPI HQMVLAICAD
     EMYEALDMAL RAGYMVENRV FEFLEPFVSA INYNLNVQRT VSSSGFRSQK GIGMSGGGGS
     GTKEQTWKYG ASMIATIKEQ VSSLISWSQN QYTGPPFGYE LVMILSSVDT LGHKYVLEQL
     IEEIALADSN GTGHYTVEVV ATIATVAFII STSVKSPRPF SDSQLILKSL DNMVNDSTGK
     SKDICVLLQS KILQLLTTAG YKPDGSRLDG KPHAKEDYSF VVNRTGEVEN KPINDSSQNS
     NTDNQFMDTN MFGGDDFTMD FGGDIDMPDL F