MED6_ARATH
ID MED6_ARATH Reviewed; 298 AA.
AC F4IXJ7; Q9LIG1;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 6;
GN Name=MED6; Synonyms=MED6_1; OrderedLocusNames=At3g21350; ORFNames=MHC9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, INTERACTION WITH MED2, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [5]
RP IDENTIFICATION, IDENTIFICATION IN THE MEDIATOR COMPLEX, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. Interacts with MED2.
CC {ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:22021418}.
CC -!- INTERACTION:
CC F4IXJ7; Q6ID77: MED11; NbExp=3; IntAct=EBI-1386187, EBI-1386244;
CC F4IXJ7; Q9SR02: MED14; NbExp=3; IntAct=EBI-1386187, EBI-1386248;
CC F4IXJ7; Q9FMP0: MED19A; NbExp=2; IntAct=EBI-1386187, EBI-1386401;
CC F4IXJ7; Q9LYW3: MED7A; NbExp=2; IntAct=EBI-1386187, EBI-1386173;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4IXJ7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4IXJ7-2; Sequence=VSP_043989;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 6 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP001305; BAB03048.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76496.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76497.1; -; Genomic_DNA.
DR EMBL; AY070104; AAL49841.1; -; mRNA.
DR EMBL; AY091238; AAM14177.1; -; mRNA.
DR RefSeq; NP_001154633.1; NM_001161161.1. [F4IXJ7-1]
DR RefSeq; NP_188772.2; NM_113030.5. [F4IXJ7-2]
DR AlphaFoldDB; F4IXJ7; -.
DR SMR; F4IXJ7; -.
DR BioGRID; 7021; 11.
DR DIP; DIP-60618N; -.
DR IntAct; F4IXJ7; 43.
DR STRING; 3702.AT3G21350.2; -.
DR PaxDb; F4IXJ7; -.
DR PRIDE; F4IXJ7; -.
DR ProteomicsDB; 228823; -. [F4IXJ7-1]
DR EnsemblPlants; AT3G21350.1; AT3G21350.1; AT3G21350. [F4IXJ7-2]
DR EnsemblPlants; AT3G21350.2; AT3G21350.2; AT3G21350. [F4IXJ7-1]
DR GeneID; 821689; -.
DR Gramene; AT3G21350.1; AT3G21350.1; AT3G21350. [F4IXJ7-2]
DR Gramene; AT3G21350.2; AT3G21350.2; AT3G21350. [F4IXJ7-1]
DR KEGG; ath:AT3G21350; -.
DR Araport; AT3G21350; -.
DR TAIR; locus:2089488; AT3G21350.
DR eggNOG; KOG3169; Eukaryota.
DR OMA; HLHNMIG; -.
DR OrthoDB; 1251252at2759; -.
DR PRO; PR:F4IXJ7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4IXJ7; baseline and differential.
DR Genevisible; F4IXJ7; AT.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.10.450.580; -; 1.
DR InterPro; IPR007018; Mediator_Med6.
DR InterPro; IPR038566; Mediator_Med6_sf.
DR PANTHER; PTHR13104; PTHR13104; 2.
DR Pfam; PF04934; Med6; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..298
FT /note="Mediator of RNA polymerase II transcription subunit
FT 6"
FT /id="PRO_0000418111"
FT REGION 245..298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 169..210
FT /note="DLQVCLVAIVLSLSVNLGSYFLLIFKLMANGEQVYGFNKFLF -> V (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_043989"
SQ SEQUENCE 298 AA; 33411 MW; 000D39D2EDD7E4E7 CRC64;
MDSSLLSAAT ADTFNGNAAD QIPPPLQPPG TDMTGISFRD QLWINSYPLD RNYIFDYFAL
SPFYDTTCNN EILRRRSIHP LDLSHLSKMT GLEYMLTDAT EPNLFVFRKQ KRDGPEKVTP
MLTYYILDGS IYQAPQLCSV FAARVSRTIY NISKAFTDAA SKLETIRQDL QVCLVAIVLS
LSVNLGSYFL LIFKLMANGE QVYGFNKFLF DTENQNEPAE SKPASETVDL KEMKRVDVIL
TSLYRKLAPP PPPPPFPEGY VSQEALGEKE ELGTQGGESQ PPQVDPIIDQ GPAKRMKF
