MED6_CAEEL
ID MED6_CAEEL Reviewed; 250 AA.
AC Q9N337; O18534;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 6;
DE AltName: Full=CeMED6;
DE AltName: Full=Mediator complex subunit 6;
DE AltName: Full=c-MED6;
DE Short=MED-6;
GN Name=mdt-6; Synonyms=med-6; ORFNames=Y57E12AL.5;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-250.
RX PubMed=9234719; DOI=10.1128/mcb.17.8.4622;
RA Lee Y.C., Min S., Gim B.S., Kim Y.-J.;
RT "A transcriptional mediator protein that is required for activation of many
RT RNA polymerase II promoters and is conserved from yeast to humans.";
RL Mol. Cell. Biol. 17:4622-4632(1997).
RN [3]
RP FUNCTION, INTERACTION WITH RNA POLYMERASE II, AND SUBCELLULAR LOCATION.
RX PubMed=10611325; DOI=10.1073/pnas.96.26.14990;
RA Kwon J.Y., Park J.M., Gim B.S., Han S.J., Lee J., Kim Y.-J.;
RT "Caenorhabditis elegans mediator complexes are required for developmental-
RT specific transcriptional activation.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:14990-14995(1999).
RN [4]
RP FUNCTION.
RX PubMed=11728440; DOI=10.1016/s0014-5793(01)03072-1;
RA Kwon J.Y., Kim-Ha J., Lee B.J., Lee J.;
RT "The MED-7 transcriptional mediator encoded by let-49 is required for gonad
RT and germ cell development in Caenorhabditis elegans.";
RL FEBS Lett. 508:305-308(2001).
RN [5]
RP FUNCTION, AND INTERACTION WITH LET-19.
RX PubMed=15790964; DOI=10.1242/dev.01776;
RA Yoda A., Kouike H., Okano H., Sawa H.;
RT "Components of the transcriptional Mediator complex are required for
RT asymmetric cell division in C. elegans.";
RL Development 132:1885-1893(2005).
RN [6]
RP INTERACTION WITH MDT-28.
RX PubMed=28603670; DOI=10.7717/peerj.3390;
RA Kostrouchova M., Kostrouch D., Chughtai A.A., Kassak F., Novotny J.P.,
RA Kostrouchova V., Benda A., Krause M.W., Saudek V., Kostrouchova M.,
RA Kostrouch Z.;
RT "The nematode homologue of Mediator complex subunit 28, F28F8.5, is a
RT critical regulator of C. elegans development.";
RL PeerJ 5:E3390-E3390(2017).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Acts to repress
CC beta-catenin target genes. Required for asymmetric division of T-cells
CC and for gonad and germ cell development. {ECO:0000250,
CC ECO:0000269|PubMed:10611325, ECO:0000269|PubMed:11728440,
CC ECO:0000269|PubMed:15790964}.
CC -!- SUBUNIT: Component of the Mediator complex (By similarity). Interacts
CC with let-19/mdt-13 (PubMed:15790964). Interacts with RNA polymerase II
CC (PubMed:10611325). Interacts with mdt-28 (PubMed:28603670).
CC {ECO:0000250|UniProtKB:O75586, ECO:0000269|PubMed:10611325,
CC ECO:0000269|PubMed:15790964, ECO:0000269|PubMed:28603670}.
CC -!- INTERACTION:
CC Q9N337; P16356: ama-1; NbExp=2; IntAct=EBI-1533827, EBI-1533906;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 6 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB84362.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FO081184; CCD69750.1; -; Genomic_DNA.
DR EMBL; U78081; AAB84362.1; ALT_INIT; mRNA.
DR PIR; T37473; T37473.
DR RefSeq; NP_504791.3; NM_072390.4.
DR AlphaFoldDB; Q9N337; -.
DR SMR; Q9N337; -.
DR BioGRID; 44136; 2.
DR IntAct; Q9N337; 4.
DR STRING; 6239.Y57E12AL.5; -.
DR EPD; Q9N337; -.
DR PaxDb; Q9N337; -.
DR PeptideAtlas; Q9N337; -.
DR PRIDE; Q9N337; -.
DR EnsemblMetazoa; Y57E12AL.5.1; Y57E12AL.5.1; WBGene00003164.
DR GeneID; 179091; -.
DR KEGG; cel:CELE_Y57E12AL.5; -.
DR UCSC; Y57E12AL.5; c. elegans.
DR CTD; 179091; -.
DR WormBase; Y57E12AL.5; CE25510; WBGene00003164; mdt-6.
DR eggNOG; KOG3169; Eukaryota.
DR GeneTree; ENSGT00390000017666; -.
DR HOGENOM; CLU_077754_1_1_1; -.
DR InParanoid; Q9N337; -.
DR OMA; FYEMNSC; -.
DR OrthoDB; 1251252at2759; -.
DR PhylomeDB; Q9N337; -.
DR PRO; PR:Q9N337; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00003164; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.10.450.580; -; 1.
DR InterPro; IPR007018; Mediator_Med6.
DR InterPro; IPR016820; Mediator_Med6_met/pln.
DR InterPro; IPR038566; Mediator_Med6_sf.
DR PANTHER; PTHR13104; PTHR13104; 1.
DR Pfam; PF04934; Med6; 1.
DR PIRSF; PIRSF023869; Mediator_MED6_meta/pln; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..250
FT /note="Mediator of RNA polymerase II transcription subunit
FT 6"
FT /id="PRO_0000303047"
FT REGION 166..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 166..186
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 211..226
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..250
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 250 AA; 29407 MW; 4AFE17BA9BDE8511 CRC64;
MMPRMGPPAA ARQDNPLHVS FRNPQWPPNF INKDNVLDYF CNQANAFYEM NSCNQQIRMQ
NIVNRTVEEC LRTMPGIQYV LWYSQPPLFI ICKQRRNNVT NVSPIAYYYV INGSVHQAPD
MYSLVQSRLL GALEPLRNAF GEVTNYSRYN TAKGYYWEFK NKPNVKKREE EKKEDEEEKL
EDRSTNFQKT RTMMLLNQLF SEMPAEDALE REEKEEVEEE EEETLKTEEP TTSTDEPKFA
EPTARTTSKQ
