MED6_DROME
ID MED6_DROME Reviewed; 249 AA.
AC Q8MSX2; Q9VH74;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 6;
DE AltName: Full=Mediator complex subunit 6;
DE AltName: Full=dMED6;
GN Name=MED6; ORFNames=CG9473;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP ASSOCIATION WITH PROMOTER REGIONS.
RX PubMed=11511356; DOI=10.1016/s1097-2765(01)00296-9;
RA Park J.M., Werner J., Kim J.M., Lis J.T., Kim Y.-J.;
RT "Mediator, not holoenzyme, is directly recruited to the heat shock promoter
RT by HSF upon heat shock.";
RL Mol. Cell 8:9-19(2001).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION OF THE MEDIATOR COMPLEX,
RP IDENTIFICATION IN A COMPLEX WITH CDK8; MED4; MED14; MED17; MED18; MED20;
RP MED21 AND MED31, AND DEVELOPMENTAL STAGE.
RX PubMed=11259581; DOI=10.1128/mcb.21.7.2312-2323.2001;
RA Park J.M., Gim B.S., Kim J.M., Yoon J.H., Kim H.-S., Kang J.-G., Kim Y.-J.;
RT "Drosophila Mediator complex is broadly utilized by diverse gene-specific
RT transcription factors at different types of core promoters.";
RL Mol. Cell. Biol. 21:2312-2323(2001).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION OF THE MEDIATOR COMPLEX,
RP IDENTIFICATION IN THE MEDIATOR COMPLEX, AND INTERACTION WITH MED17; MED18;
RP MED20; MED21; MED23; MED24; MED27 AND MED30.
RX PubMed=12021283; DOI=10.1074/jbc.m204144200;
RA Gu J.-Y., Park J.M., Song E.J., Mizuguchi G., Yoon J.H., Kim-Ha J.,
RA Lee K.-J., Kim Y.-J.;
RT "Novel Mediator proteins of the small Mediator complex in Drosophila SL2
RT cells.";
RL J. Biol. Chem. 277:27154-27161(2002).
RN [7]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. Required for activated
CC transcription of the MtnA, MtnB and MtnD genes.
CC {ECO:0000269|PubMed:11259581, ECO:0000269|PubMed:12021283,
CC ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex, which includes at least
CC MED4, MED6, MED14, MED17, MED18, MED20, MED21, MED23, MED24, MED27,
CC MED30 and MED31. {ECO:0000269|PubMed:11259581,
CC ECO:0000269|PubMed:12021283}.
CC -!- INTERACTION:
CC Q8MSX2; Q9VEC1: MED17; NbExp=6; IntAct=EBI-194467, EBI-135284;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Maternally encoded. Expression decreases during
CC larval stages then rises during mid-pupal metamorphosis.
CC {ECO:0000269|PubMed:11259581}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 6 family.
CC {ECO:0000305}.
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DR EMBL; AE014297; AAF54445.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN13445.2; -; Genomic_DNA.
DR EMBL; AY118517; AAM49886.1; -; mRNA.
DR EMBL; BT001633; AAN71388.1; -; mRNA.
DR RefSeq; NP_001163577.1; NM_001170106.1.
DR RefSeq; NP_001163578.1; NM_001170107.1.
DR RefSeq; NP_001287264.1; NM_001300335.1.
DR RefSeq; NP_651988.2; NM_143731.6.
DR RefSeq; NP_731403.2; NM_169310.2.
DR AlphaFoldDB; Q8MSX2; -.
DR SMR; Q8MSX2; -.
DR BioGRID; 69231; 37.
DR IntAct; Q8MSX2; 13.
DR STRING; 7227.FBpp0081639; -.
DR PaxDb; Q8MSX2; -.
DR PRIDE; Q8MSX2; -.
DR DNASU; 44728; -.
DR EnsemblMetazoa; FBtr0082161; FBpp0081639; FBgn0024330.
DR EnsemblMetazoa; FBtr0082162; FBpp0081640; FBgn0024330.
DR EnsemblMetazoa; FBtr0301872; FBpp0291086; FBgn0024330.
DR EnsemblMetazoa; FBtr0301873; FBpp0291087; FBgn0024330.
DR EnsemblMetazoa; FBtr0339719; FBpp0308776; FBgn0024330.
DR GeneID; 44728; -.
DR KEGG; dme:Dmel_CG9473; -.
DR CTD; 10001; -.
DR FlyBase; FBgn0024330; MED6.
DR VEuPathDB; VectorBase:FBgn0024330; -.
DR eggNOG; KOG3169; Eukaryota.
DR GeneTree; ENSGT00390000017666; -.
DR HOGENOM; CLU_077754_1_1_1; -.
DR InParanoid; Q8MSX2; -.
DR OMA; HLHNMIG; -.
DR OrthoDB; 1251252at2759; -.
DR PhylomeDB; Q8MSX2; -.
DR BioGRID-ORCS; 44728; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 44728; -.
DR PRO; PR:Q8MSX2; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0024330; Expressed in wing disc and 35 other tissues.
DR ExpressionAtlas; Q8MSX2; baseline and differential.
DR Genevisible; Q8MSX2; DM.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0016592; C:mediator complex; IDA:FlyBase.
DR GO; GO:0005654; C:nucleoplasm; ISS:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; HMP:FlyBase.
DR GO; GO:0045824; P:negative regulation of innate immune response; HMP:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:GOC.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR Gene3D; 3.10.450.580; -; 1.
DR InterPro; IPR007018; Mediator_Med6.
DR InterPro; IPR016820; Mediator_Med6_met/pln.
DR InterPro; IPR038566; Mediator_Med6_sf.
DR PANTHER; PTHR13104; PTHR13104; 1.
DR Pfam; PF04934; Med6; 1.
DR PIRSF; PIRSF023869; Mediator_MED6_meta/pln; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..249
FT /note="Mediator of RNA polymerase II transcription subunit
FT 6"
FT /id="PRO_0000303045"
FT REGION 156..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 198..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 156..170
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 209..229
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 249 AA; 28374 MW; C5667F1A53E7F54A CRC64;
MASRQMTNDH LRLSWHDTQM MATLSPQTVM DYFCRKSNPF YDHMCNNETV RMQRLGPEHL
HNMIGLEYIL LHVAEPILYV IRKQHRHNPS EATPIADYYI IGGTVYKAPD LANVINSRIL
NTVVNLQSAF EEASSYARYH PNKGYTWDFS SNKVFSDRSK SDKKDANSAK DENSGTLFQK
QRVDMLLAEL LRKFPPPIPP MLQNLQQPPP AGDDLNTARN ASEMNNATGP LDIKTEGVDM
KPPPEKKSK
