MED6_SCHPO
ID MED6_SCHPO Reviewed; 216 AA.
AC Q9US45;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 6;
DE AltName: Full=Mediator complex subunit 6;
DE AltName: Full=RNA polymerase II mediator complex protein pmc5;
GN Name=med6; Synonyms=pmc5; ORFNames=SPAC1002.15c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RC STRAIN=972 / ATCC 24843;
RX PubMed=10625684; DOI=10.1074/jbc.275.2.1351;
RA Spaehr H., Beve J., Larsson T., Bergstroem J., Karlsson K.-A.,
RA Gustafsson C.M.;
RT "Purification and characterization of RNA polymerase II holoenzyme from
RT Schizosaccharomyces pombe.";
RL J. Biol. Chem. 275:1351-1356(2000).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RX PubMed=11572939; DOI=10.1073/pnas.211253898;
RA Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT subunits conserved between yeast and metazoan cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:10625684, ECO:0000269|PubMed:11572939}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 6 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CU329670; CAB65615.1; -; Genomic_DNA.
DR RefSeq; NP_593503.1; NM_001018937.2.
DR PDB; 4H61; X-ray; 2.70 A; A/B=10-180.
DR PDB; 4H63; X-ray; 3.40 A; F=1-180.
DR PDB; 5N9J; X-ray; 3.40 A; S=1-216.
DR PDB; 5U0P; EM; 4.40 A; F=1-216.
DR PDB; 5U0S; EM; 7.80 A; F=1-216.
DR PDBsum; 4H61; -.
DR PDBsum; 4H63; -.
DR PDBsum; 5N9J; -.
DR PDBsum; 5U0P; -.
DR PDBsum; 5U0S; -.
DR AlphaFoldDB; Q9US45; -.
DR SMR; Q9US45; -.
DR BioGRID; 279691; 109.
DR DIP; DIP-38748N; -.
DR IntAct; Q9US45; 7.
DR STRING; 4896.SPAC1002.15c.1; -.
DR MaxQB; Q9US45; -.
DR PaxDb; Q9US45; -.
DR EnsemblFungi; SPAC1002.15c.1; SPAC1002.15c.1:pep; SPAC1002.15c.
DR GeneID; 2543263; -.
DR KEGG; spo:SPAC1002.15c; -.
DR PomBase; SPAC1002.15c; med6.
DR VEuPathDB; FungiDB:SPAC1002.15c; -.
DR eggNOG; KOG3169; Eukaryota.
DR HOGENOM; CLU_077754_6_0_1; -.
DR InParanoid; Q9US45; -.
DR OMA; CNENIYM; -.
DR PhylomeDB; Q9US45; -.
DR PRO; PR:Q9US45; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:PomBase.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:PomBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.10.450.580; -; 1.
DR InterPro; IPR007018; Mediator_Med6.
DR InterPro; IPR016612; Mediator_Med6_fun.
DR InterPro; IPR038566; Mediator_Med6_sf.
DR PANTHER; PTHR13104; PTHR13104; 1.
DR Pfam; PF04934; Med6; 1.
DR PIRSF; PIRSF013286; MED6_fungi; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..216
FT /note="Mediator of RNA polymerase II transcription subunit
FT 6"
FT /id="PRO_0000096390"
FT REGION 157..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 11..13
FT /evidence="ECO:0007829|PDB:4H61"
FT HELIX 19..24
FT /evidence="ECO:0007829|PDB:4H61"
FT TURN 30..32
FT /evidence="ECO:0007829|PDB:4H61"
FT HELIX 33..38
FT /evidence="ECO:0007829|PDB:4H61"
FT STRAND 40..43
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 48..58
FT /evidence="ECO:0007829|PDB:4H61"
FT HELIX 66..71
FT /evidence="ECO:0007829|PDB:4H61"
FT STRAND 75..84
FT /evidence="ECO:0007829|PDB:4H61"
FT TURN 85..87
FT /evidence="ECO:0007829|PDB:4H61"
FT STRAND 88..96
FT /evidence="ECO:0007829|PDB:4H61"
FT STRAND 98..100
FT /evidence="ECO:0007829|PDB:4H61"
FT STRAND 102..111
FT /evidence="ECO:0007829|PDB:4H61"
FT STRAND 114..117
FT /evidence="ECO:0007829|PDB:4H61"
FT HELIX 120..140
FT /evidence="ECO:0007829|PDB:4H61"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:4H63"
FT TURN 151..153
FT /evidence="ECO:0007829|PDB:4H63"
FT STRAND 154..156
FT /evidence="ECO:0007829|PDB:4H63"
FT HELIX 193..211
FT /evidence="ECO:0007829|PDB:5N9J"
SQ SEQUENCE 216 AA; 24906 MW; 84AE0C11F607F35A CRC64;
MASGAPPSVD LTSIQWRMPE WVQSMGGLRT ENVLEYFSQS PFYSHKSNNE MLKMQSQFNA
LDLGDLNSQL KRLTGIQFVI IHERPPFLWV IQKQNRLNEN EVKPLTVYFV CNENIYMAPN
AYTLLATRML NATYCFQKAL TKIEKFPQYN PQEGYTYPKL SNDNLEVDHS NTNEPADENK
NQSIENADYS FSPEDFSVVR AFMQSLHSSK EAPDVK
