MED7B_ARATH
ID MED7B_ARATH Reviewed; 168 AA.
AC Q9LZD7;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 7b;
GN Name=MED7B; Synonyms=MED7_2; OrderedLocusNames=At5g03500;
GN ORFNames=F12E4.270;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [6]
RP IDENTIFICATION, SUBUNIT, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [7]
RP INTERACTION WITH ME14/CBP1.
RX PubMed=26462908; DOI=10.1105/tpc.15.00370;
RA Li H.J., Zhu S.S., Zhang M.X., Wang T., Liang L., Xue Y., Shi D.Q., Liu J.,
RA Yang W.C.;
RT "Arabidopsis CBP1 is a novel regulator of transcription initiation in
RT central cell-mediated pollen tube guidance.";
RL Plant Cell 27:2880-2893(2015).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional pre-initiation complex with RNA polymerase II and the
CC general transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex (PubMed:17560376,
CC PubMed:22021418). Interacts with MEE14/CBP1 (PubMed:26462908).
CC {ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:22021418,
CC ECO:0000269|PubMed:26462908}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 7 family.
CC {ECO:0000305}.
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DR EMBL; AL162751; CAB83310.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90613.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90614.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90615.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69262.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69263.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69265.1; -; Genomic_DNA.
DR EMBL; AY075596; AAL91616.1; -; mRNA.
DR EMBL; AY097400; AAM19916.1; -; mRNA.
DR EMBL; AK317313; BAH19989.1; -; mRNA.
DR PIR; T48375; T48375.
DR RefSeq; NP_001078525.1; NM_001085056.2.
DR RefSeq; NP_001190215.1; NM_001203286.1.
DR RefSeq; NP_001330956.1; NM_001342707.1.
DR RefSeq; NP_001330957.1; NM_001342708.1.
DR RefSeq; NP_001330959.1; NM_001342710.1.
DR RefSeq; NP_195970.2; NM_120430.3.
DR AlphaFoldDB; Q9LZD7; -.
DR SMR; Q9LZD7; -.
DR BioGRID; 17098; 10.
DR IntAct; Q9LZD7; 10.
DR STRING; 3702.AT5G03500.3; -.
DR PaxDb; Q9LZD7; -.
DR PRIDE; Q9LZD7; -.
DR EnsemblPlants; AT5G03500.1; AT5G03500.1; AT5G03500.
DR EnsemblPlants; AT5G03500.2; AT5G03500.2; AT5G03500.
DR EnsemblPlants; AT5G03500.3; AT5G03500.3; AT5G03500.
DR EnsemblPlants; AT5G03500.4; AT5G03500.4; AT5G03500.
DR EnsemblPlants; AT5G03500.5; AT5G03500.5; AT5G03500.
DR EnsemblPlants; AT5G03500.7; AT5G03500.7; AT5G03500.
DR GeneID; 831822; -.
DR Gramene; AT5G03500.1; AT5G03500.1; AT5G03500.
DR Gramene; AT5G03500.2; AT5G03500.2; AT5G03500.
DR Gramene; AT5G03500.3; AT5G03500.3; AT5G03500.
DR Gramene; AT5G03500.4; AT5G03500.4; AT5G03500.
DR Gramene; AT5G03500.5; AT5G03500.5; AT5G03500.
DR Gramene; AT5G03500.7; AT5G03500.7; AT5G03500.
DR KEGG; ath:AT5G03500; -.
DR Araport; AT5G03500; -.
DR TAIR; locus:2831839; AT5G03500.
DR eggNOG; KOG0570; Eukaryota.
DR HOGENOM; CLU_065214_4_0_1; -.
DR InParanoid; Q9LZD7; -.
DR OMA; HTIFINM; -.
DR PhylomeDB; Q9LZD7; -.
DR PRO; PR:Q9LZD7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LZD7; baseline and differential.
DR Genevisible; Q9LZD7; AT.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0009704; P:de-etiolation; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0051510; P:regulation of unidimensional cell growth; IMP:TAIR.
DR Gene3D; 6.10.140.200; -; 1.
DR InterPro; IPR037212; Med7/Med21-like.
DR InterPro; IPR009244; Mediatior_Med7.
DR InterPro; IPR044888; Mediatior_Med7_sf.
DR PANTHER; PTHR21428; PTHR21428; 1.
DR Pfam; PF05983; Med7; 1.
DR SUPFAM; SSF140718; SSF140718; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..168
FT /note="Mediator of RNA polymerase II transcription subunit
FT 7b"
FT /id="PRO_0000418113"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 64..92
FT /evidence="ECO:0000255"
FT COILED 132..162
FT /evidence="ECO:0000255"
SQ SEQUENCE 168 AA; 19299 MW; 3E94164909CC42D9 CRC64;
MATATYPPPP PYYRLYKDFS ENTDSAPEPP PPIEGTYVCF GGNYTTEDVL PSLEEQGVPQ
LYPKDSNLDY KKELRSLNRE LQLHILELAD VLVDRPSQYA KRIGEISSIF KNLHHLLNSL
RPHQARATLI HIMELQIQQR KQAVEDIKRR REEAQGLLKD AFVTLDGQ
