MED7_CAEEL
ID MED7_CAEEL Reviewed; 251 AA.
AC Q95Q17; Q9XWF7;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 7;
DE AltName: Full=CeMED7;
DE Short=MED-7;
DE AltName: Full=Lethal protein 49;
DE AltName: Full=Mediator complex subunit 7;
GN Name=let-49; Synonyms=mdt-7, med-7; ORFNames=Y54E5B.3;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND INTERACTION WITH MDT-10; MDT-21 AND RNA POLYMERASE II.
RX PubMed=10611325; DOI=10.1073/pnas.96.26.14990;
RA Kwon J.Y., Park J.M., Gim B.S., Han S.J., Lee J., Kim Y.-J.;
RT "Caenorhabditis elegans mediator complexes are required for developmental-
RT specific transcriptional activation.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:14990-14995(1999).
RN [3]
RP FUNCTION.
RX PubMed=11728440; DOI=10.1016/s0014-5793(01)03072-1;
RA Kwon J.Y., Kim-Ha J., Lee B.J., Lee J.;
RT "The MED-7 transcriptional mediator encoded by let-49 is required for gonad
RT and germ cell development in Caenorhabditis elegans.";
RL FEBS Lett. 508:305-308(2001).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC germ cell development and gonadal growth. {ECO:0000250,
CC ECO:0000269|PubMed:10611325, ECO:0000269|PubMed:11728440}.
CC -!- SUBUNIT: Component of the Mediator complex (By similarity). Interacts
CC with mdt-10 and mdt-21. Interacts with RNA polymerase II. {ECO:0000250,
CC ECO:0000269|PubMed:10611325}.
CC -!- INTERACTION:
CC Q95Q17; Q6BER6: mdt-21; NbExp=2; IntAct=EBI-1533863, EBI-1533858;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b;
CC IsoId=Q95Q17-1; Sequence=Displayed;
CC Name=a;
CC IsoId=Q95Q17-2; Sequence=VSP_028004;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 7 family.
CC {ECO:0000305}.
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DR EMBL; AL032653; CAA21715.1; -; Genomic_DNA.
DR EMBL; AL032653; CAC42382.1; -; Genomic_DNA.
DR PIR; T27166; T27166.
DR RefSeq; NP_493585.1; NM_061184.3. [Q95Q17-2]
DR RefSeq; NP_493586.1; NM_061185.6. [Q95Q17-1]
DR AlphaFoldDB; Q95Q17; -.
DR SMR; Q95Q17; -.
DR BioGRID; 38736; 3.
DR IntAct; Q95Q17; 5.
DR STRING; 6239.Y54E5B.3a; -.
DR EPD; Q95Q17; -.
DR PaxDb; Q95Q17; -.
DR PeptideAtlas; Q95Q17; -.
DR EnsemblMetazoa; Y54E5B.3a.1; Y54E5B.3a.1; WBGene00002324. [Q95Q17-2]
DR EnsemblMetazoa; Y54E5B.3b.1; Y54E5B.3b.1; WBGene00002324. [Q95Q17-1]
DR GeneID; 173353; -.
DR KEGG; cel:CELE_Y54E5B.3; -.
DR UCSC; Y54E5B.3b; c. elegans. [Q95Q17-1]
DR CTD; 173353; -.
DR WormBase; Y54E5B.3a; CE19227; WBGene00002324; let-49. [Q95Q17-2]
DR WormBase; Y54E5B.3b; CE28136; WBGene00002324; let-49. [Q95Q17-1]
DR eggNOG; KOG0570; Eukaryota.
DR GeneTree; ENSGT00940000165241; -.
DR InParanoid; Q95Q17; -.
DR OMA; HTIFINM; -.
DR OrthoDB; 1340410at2759; -.
DR PhylomeDB; Q95Q17; -.
DR PRO; PR:Q95Q17; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00002324; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IGI:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0000003; P:reproduction; IMP:WormBase.
DR Gene3D; 6.10.140.200; -; 1.
DR InterPro; IPR037212; Med7/Med21-like.
DR InterPro; IPR009244; Mediatior_Med7.
DR InterPro; IPR044888; Mediatior_Med7_sf.
DR PANTHER; PTHR21428; PTHR21428; 1.
DR Pfam; PF05983; Med7; 1.
DR SUPFAM; SSF140718; SSF140718; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Developmental protein; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..251
FT /note="Mediator of RNA polymerase II transcription subunit
FT 7"
FT /id="PRO_0000303186"
FT REGION 1..38
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 30
FT /note="N -> NDS (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_028004"
SQ SEQUENCE 251 AA; 28888 MW; 22E28653EB687721 CRC64;
MLPGFGAQTV SPFPNPPEYA SAYTSDRINN GSAPPPPHPL TEFKVYGEEY RLEDDVIAPL
KNAGVAELYK NKNNWKTEMK KLNRSAIVAF FDLVEILIRA PDHPMREEKM VDLHTIFINM
HHLINEFRPV QARDSVRILQ ERQIEELSDI CKDFKKYLRD GREVVDDQFQ MIRGKLPAPP
QPSELTRVKL QDGVLHMLQE TEASDDVEMK EEEGSEYSKK KARLELLSRE DGPPSVVHLL
ARQFHDISLK K
