MED7_SCHPO
ID MED7_SCHPO Reviewed; 376 AA.
AC O60104; Q9US98;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 7;
DE AltName: Full=Mediator complex subunit 7;
GN Name=med7; ORFNames=SPBC14F5.08;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 176-374, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=ATCC 38364 / 968;
RX PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA Hiraoka Y.;
RT "Large-scale screening of intracellular protein localization in living
RT fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL Genes Cells 5:169-190(2000).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RC STRAIN=972 / ATCC 24843;
RX PubMed=10625684; DOI=10.1074/jbc.275.2.1351;
RA Spaehr H., Beve J., Larsson T., Bergstroem J., Karlsson K.-A.,
RA Gustafsson C.M.;
RT "Purification and characterization of RNA polymerase II holoenzyme from
RT Schizosaccharomyces pombe.";
RL J. Biol. Chem. 275:1351-1356(2000).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR
RP COMPLEX.
RX PubMed=11572939; DOI=10.1073/pnas.211253898;
RA Spaehr H., Samuelsen C.O., Baraznenok V., Ernest I., Huylebroeck D.,
RA Remacle J.E., Samuelsson T., Kieselbach T., Holmberg S., Gustafsson C.M.;
RT "Analysis of Schizosaccharomyces pombe mediator reveals a set of essential
RT subunits conserved between yeast and metazoan cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11985-11990(2001).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=16630887; DOI=10.1016/j.molcel.2006.03.032;
RA Zhu X., Wiren M., Sinha I., Rasmussen N.N., Linder T., Holmberg S.,
RA Ekwall K., Gustafsson C.M.;
RT "Genome-wide occupancy profile of mediator and the Srb8-11 module reveals
RT interactions with coding regions.";
RL Mol. Cell 22:169-178(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10759889,
CC ECO:0000269|PubMed:16630887}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 7 family.
CC {ECO:0000305}.
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DR EMBL; CU329671; CAA19326.1; -; Genomic_DNA.
DR EMBL; AB027928; BAA87232.1; -; Genomic_DNA.
DR PIR; T39454; T39454.
DR RefSeq; NP_596734.1; NM_001022660.2.
DR PDB; 5N9J; X-ray; 3.40 A; E=1-376.
DR PDB; 5U0P; EM; 4.40 A; G=1-376.
DR PDB; 5U0S; EM; 7.80 A; G=1-376.
DR PDBsum; 5N9J; -.
DR PDBsum; 5U0P; -.
DR PDBsum; 5U0S; -.
DR AlphaFoldDB; O60104; -.
DR SMR; O60104; -.
DR BioGRID; 276324; 75.
DR IntAct; O60104; 1.
DR STRING; 4896.SPBC14F5.08.1; -.
DR iPTMnet; O60104; -.
DR MaxQB; O60104; -.
DR PaxDb; O60104; -.
DR PRIDE; O60104; -.
DR EnsemblFungi; SPBC14F5.08.1; SPBC14F5.08.1:pep; SPBC14F5.08.
DR GeneID; 2539773; -.
DR KEGG; spo:SPBC14F5.08; -.
DR PomBase; SPBC14F5.08; med7.
DR VEuPathDB; FungiDB:SPBC14F5.08; -.
DR eggNOG; KOG0570; Eukaryota.
DR HOGENOM; CLU_774237_0_0_1; -.
DR InParanoid; O60104; -.
DR OMA; FGDTWRL; -.
DR PhylomeDB; O60104; -.
DR PRO; PR:O60104; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0000791; C:euchromatin; IDA:PomBase.
DR GO; GO:0016592; C:mediator complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:PomBase.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:PomBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 6.10.140.200; -; 1.
DR InterPro; IPR037212; Med7/Med21-like.
DR InterPro; IPR009244; Mediatior_Med7.
DR InterPro; IPR044888; Mediatior_Med7_sf.
DR PANTHER; PTHR21428; PTHR21428; 1.
DR Pfam; PF05983; Med7; 1.
DR SUPFAM; SSF140718; SSF140718; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..376
FT /note="Mediator of RNA polymerase II transcription subunit
FT 7"
FT /id="PRO_0000096392"
FT REGION 138..215
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 336..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..186
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 339..358
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 18..22
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 25..37
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 56..64
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 192..195
FT /evidence="ECO:0007829|PDB:5N9J"
FT STRAND 211..214
FT /evidence="ECO:0007829|PDB:5N9J"
FT TURN 215..218
FT /evidence="ECO:0007829|PDB:5N9J"
FT STRAND 219..222
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 223..248
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 250..252
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 253..314
FT /evidence="ECO:0007829|PDB:5N9J"
FT HELIX 322..334
FT /evidence="ECO:0007829|PDB:5N9J"
SQ SEQUENCE 376 AA; 43509 MW; A2FFA4E9CFAAEE99 CRC64;
MEPNEGVQLF SAFPPPPPYY KLFTRENIEK VISNMEKEAK HEDDANTLQP KTEEEIESLA
KLFKKPSCLT SGTYQMFGDT WRLDEAIPSL KEFGIPELYK DIKDGEDEIE VVEYDPKSNA
IVGTSFTRVH DYDKNSPIEN EKILEDDQHT AMKEKDNESD KTMKDVEEKT EPSLKKEEED
IQMKEPLDSQ DTGAVSASSV NEGFRADQKS KDGETSDLIK IPRRAYELRF LSRSLMLNFL
ELLGIMAKAP EQFPSKVENI RVLLLNLHHL INDYRPHQSR ESLIMLLEKQ LKHEESQVEL
LRTHNRQMTE TLEKYKSLDF NMEKEGDVIQ QLKSSIKKPL SGAEDEQKSR SMFSKNDEKL
KKSLELMEDV IKRDLS
