ID   MED8A_XENLA             Reviewed;         268 AA.
AC   Q5HZZ6;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8-A;
DE   AltName: Full=Mediator complex subunit 8-A;
GN   Name=med8-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors. May play a role as a target
CC       recruitment subunit in E3 ubiquitin-protein ligase complexes and thus
CC       in ubiquitination and subsequent proteasomal degradation of target
CC       proteins (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Component of the Mediator complex. May be part of a
CC       multisubunit E3 ubiquitin-protein ligase complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000305}.
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DR   EMBL; BC088827; AAH88827.1; -; mRNA.
DR   RefSeq; NP_001088933.1; NM_001095464.1.
DR   AlphaFoldDB; Q5HZZ6; -.
DR   SMR; Q5HZZ6; -.
DR   DNASU; 496309; -.
DR   GeneID; 496309; -.
DR   KEGG; xla:496309; -.
DR   CTD; 496309; -.
DR   Xenbase; XB-GENE-963504; med8.L.
DR   OrthoDB; 1392867at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000186698; Chromosome 4L.
DR   Bgee; 496309; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; PTHR13074; 1.
DR   Pfam; PF10232; Med8; 1.
PE   2: Evidence at transcript level;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Ubl conjugation pathway.
FT   CHAIN           1..268
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   8-A"
FT                   /id="PRO_0000304526"
FT   REGION          190..268
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          1..26
FT                   /evidence="ECO:0000255"
FT   COILED          117..160
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        221..268
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   268 AA;  29535 MW;  316EF92A4BDD18C9 CRC64;
     MQREEKQLEA CLDALISQVS DIKNSLVGFI HKLENEFDRL TWPSVLDSFA LLSGQLNTLN
     KVLKNEKTPL LRNQVIIPLL LSPDRDEEIM RLTEGRVPVF SHEVVPDHLR TKPDPDVEEL
     EKQLSAEAAR ITTEAAQKQV QSMNKMCSNL LDKISKEERE SELGSLRQNK QTFNPTDTNA
     LVAAVAFGKG LSNRRPPGQG GPMAPGQTGA SGMLPSAAGM QQVPMSLQSN QQQQHMAGVS
     MSQGNQPGKM PSSIKTNIKS ASMHPYQR