MED8_ARATH
ID MED8_ARATH Reviewed; 524 AA.
AC Q4V3C1; O80620;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE AltName: Full=Protein SETH 10;
GN Name=MED8; Synonyms=MED8_1, SETH10; OrderedLocusNames=At2g03070;
GN ORFNames=T17M13.24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=15342534; DOI=10.1534/genetics.104.030270;
RA Lalanne E., Michaelidis C., Moore J.M., Gagliano W., Johnson A., Patel R.,
RA Howden R., Vielle-Calzada J.P., Grossniklaus U., Twell D.;
RT "Analysis of transposon insertion mutants highlights the diversity of
RT mechanisms underlying male progamic development in Arabidopsis.";
RL Genetics 167:1975-1986(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19671879; DOI=10.1105/tpc.109.066910;
RA Kidd B.N., Edgar C.I., Kumar K.K., Aitken E.A., Schenk P.M., Manners J.M.,
RA Kazan K.;
RT "The mediator complex subunit PFT1 is a key regulator of jasmonate-
RT dependent defense in Arabidopsis.";
RL Plant Cell 21:2237-2252(2009).
RN [8]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors. Regulator of both plant defense and flowering
CC time. Involved in pollen tube growth. {ECO:0000269|PubMed:15342534,
CC ECO:0000269|PubMed:19671879}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Increased number of leaves and shorter petioles
CC under vegetative conditions. Late-flowering phenotype when grown under
CC both long-day and short-day conditions. Increased sensitivity to
CC necrotrophic pathogens. {ECO:0000269|PubMed:19671879}.
CC -!- MISCELLANEOUS: Named seth after the brother and murderer of the
CC Egyptian fertility god Osiris.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC32925.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004138; AAC32925.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05662.1; -; Genomic_DNA.
DR EMBL; BT023435; AAY56426.1; -; mRNA.
DR EMBL; AK229348; BAF01211.1; -; mRNA.
DR PIR; A84444; A84444.
DR RefSeq; NP_178407.2; NM_126359.4.
DR AlphaFoldDB; Q4V3C1; -.
DR BioGRID; 238; 10.
DR IntAct; Q4V3C1; 1.
DR STRING; 3702.AT2G03070.1; -.
DR iPTMnet; Q4V3C1; -.
DR PaxDb; Q4V3C1; -.
DR PRIDE; Q4V3C1; -.
DR ProteomicsDB; 228827; -.
DR EnsemblPlants; AT2G03070.1; AT2G03070.1; AT2G03070.
DR GeneID; 814836; -.
DR Gramene; AT2G03070.1; AT2G03070.1; AT2G03070.
DR KEGG; ath:AT2G03070; -.
DR Araport; AT2G03070; -.
DR TAIR; locus:2056770; AT2G03070.
DR eggNOG; ENOG502QTT9; Eukaryota.
DR HOGENOM; CLU_037260_0_0_1; -.
DR InParanoid; Q4V3C1; -.
DR OMA; GYGNMQT; -.
DR OrthoDB; 747489at2759; -.
DR PhylomeDB; Q4V3C1; -.
DR PRO; PR:Q4V3C1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q4V3C1; baseline and differential.
DR Genevisible; Q4V3C1; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR InterPro; IPR038795; MED8_plant.
DR PANTHER; PTHR35552; PTHR35552; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..524
FT /note="Mediator of RNA polymerase II transcription subunit
FT 8"
FT /id="PRO_0000418346"
FT REGION 291..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 474..524
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 137..162
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 524 AA; 58157 MW; 8DB534DFA531189A CRC64;
METQPQQPPP PPVAEKLNPK LEKELNLESL KTRAVSLAKA IARILEDFDA YGRTNTTPKW
QDILGQYSMV NLELFNIVEE VKRVSNAFVV LPKNVNAMNA AILPVMLSSK LLPEMETDDN
AKREQLLQGV QSLPIPMQIE RLKARMDMIA AACENAERVL ADTRKAYGFG TRQGPSMLPT
MDKGQAAKIQ EQEKMLRDAV NDGKGTQLPP DQRQITTALP PHLADVLIIN DAGKIALPGQ
SNNINNQGMM QVSGTQFVGR SAASPSGPNF DNTTSPLPYS NSPRATGMVN VPSPQHQIQQ
QQFQQQQQRS KLMQLPQHQQ QQLLAQQQQQ LRQSSMQGLG QSQIPALHDM HGQAQQKFQT
SHGQHQMPYS QPMGAHQQFQ ARQLSGGHIQ HSMSQGQLNP AMNRHLNQFS GGANSALFTS
AQGSPSSQMI PNMSSMQSQT LVPRMQQFGV SGTNPQRSHS SQMLGDQMFN TSGMMQTQQT
QIQQSQQQQQ QQQQGGYGNM QTNQQSLQPN NMMQNAQQRH QNPQ
