ID   MED8_ASPFU              Reviewed;         249 AA.
AC   Q4WP83;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE   AltName: Full=Mediator complex subunit 8;
GN   Name=med8; ORFNames=AFUA_4G08360;
OS   Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS   A1100) (Aspergillus fumigatus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=330879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX   PubMed=16372009; DOI=10.1038/nature04332;
RA   Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA   Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA   Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA   Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA   Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA   Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA   Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA   Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA   Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA   Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA   O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA   Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA   Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA   Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA   Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA   Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA   Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA   Barrell B.G., Denning D.W.;
RT   "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT   Aspergillus fumigatus.";
RL   Nature 438:1151-1156(2005).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000305}.
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DR   EMBL; AAHF01000005; EAL89951.1; -; Genomic_DNA.
DR   RefSeq; XP_751989.1; XM_746896.1.
DR   AlphaFoldDB; Q4WP83; -.
DR   SMR; Q4WP83; -.
DR   STRING; 746128.CADAFUBP00006372; -.
DR   EnsemblFungi; EAL89951; EAL89951; AFUA_4G08360.
DR   GeneID; 3509177; -.
DR   KEGG; afm:AFUA_4G08360; -.
DR   VEuPathDB; FungiDB:Afu4g08360; -.
DR   eggNOG; ENOG502S8U1; Eukaryota.
DR   HOGENOM; CLU_074399_1_0_1; -.
DR   InParanoid; Q4WP83; -.
DR   OMA; WAPIEAN; -.
DR   OrthoDB; 1472120at2759; -.
DR   Proteomes; UP000002530; Chromosome 4.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; PTHR13074; 1.
DR   Pfam; PF10232; Med8; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..249
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   8"
FT                   /id="PRO_0000304535"
FT   REGION          170..249
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          154..200
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        176..190
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   249 AA;  27465 MW;  4FB8BEBEA521B747 CRC64;
     MASLNQDQIK ILEQSRQRLV QLTRSLASLI GSLNQSDPLP SWSSLQSQAG IISNNLVSIS
     EHLADNKDLL SALVAYPGPS YPGRTQAPTL EQLLRTKLDP RVEDWVSRGR RAGASALEDR
     GALSESALAE LWDWAPVEAN QEARRRNWGG NFTLEEREMG IQNVVTGLRR QLEDEDEEAS
     ESEEEVEEEE MEVVGVRRRS GAGAGLEFDI AAPAPGSRQQ QQQQKAAGPA VPLEDILRYM
     TTGIPPTQR