ID   MED8_CAEBR              Reviewed;         294 AA.
AC   P0C5E6; A8WTC1; E3CTW6; Q620C1;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-FEB-2010, sequence version 2.
DT   25-MAY-2022, entry version 57.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE   AltName: Full=Mediator complex subunit 8;
GN   ORFNames=CBG29108 {ECO:0000312|WormBase:CBG29108};
OS   Caenorhabditis briggsae.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6238;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AF16;
RX   PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA   Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA   Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA   Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA   Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA   Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA   Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA   Durbin R.M., Waterston R.H.;
RT   "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT   genomics.";
RL   PLoS Biol. 1:166-192(2003).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000305}.
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DR   EMBL; HE601438; CBX33037.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0C5E6; -.
DR   SMR; P0C5E6; -.
DR   STRING; 6238.CBG29108; -.
DR   WormBase; CBG29108; CBP47985; WBGene00194889; -.
DR   eggNOG; KOG3583; Eukaryota.
DR   HOGENOM; CLU_799832_0_0_1; -.
DR   InParanoid; P0C5E6; -.
DR   OMA; QEEVEWP; -.
DR   Proteomes; UP000008549; Chromosome II.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; PTHR13074; 1.
DR   Pfam; PF10232; Med8; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..294
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   8"
FT                   /id="PRO_0000304610"
FT   REGION          216..249
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          262..294
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        229..249
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   294 AA;  32869 MW;  4E13F0186F373A05 CRC64;
     MDFPFQAPIP PLTANYQSEP EKIGQATDMM IKRVTDAKKM IEELLQMLDL QEKCPWPDML
     EKFSSLASAM ASLQTSVRKS GLPHGHEDHG QFLRSHVLVP QRLQYEPDEA LLRVTQGRVF
     SWNHELVPEY LRTKPNPEME NEEQMLDMER SAKAPDLVVK QIAAYNKNIE GLLGSLSNID
     RLHSEAIIEK PTHSREETMK LVKSILLGEA IRTLRTAGAP PTSTPMPAAP GSAGPSQSGS
     QVMNQAPGMQ DYQNPQLRQQ LMGQPPQMGY GNAYPPQYPQ MHPQQMGELS GNSF