MED8_CAEEL
ID MED8_CAEEL Reviewed; 297 AA.
AC Q9U1W2; Q9U1W3;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE AltName: Full=Mediator complex subunit 8;
GN Name=mdt-8; ORFNames=Y62F5A.1;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b;
CC IsoId=Q9U1W2-1; Sequence=Displayed;
CC Name=a;
CC IsoId=Q9U1W2-2; Sequence=VSP_028028;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC {ECO:0000305}.
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DR EMBL; AL110499; CAB57912.1; -; Genomic_DNA.
DR EMBL; AL110499; CAB57913.1; -; Genomic_DNA.
DR PIR; T31474; T31474.
DR RefSeq; NP_496463.2; NM_064062.4. [Q9U1W2-1]
DR RefSeq; NP_496464.2; NM_064063.4. [Q9U1W2-2]
DR AlphaFoldDB; Q9U1W2; -.
DR SMR; Q9U1W2; -.
DR BioGRID; 40070; 37.
DR DIP; DIP-25584N; -.
DR IntAct; Q9U1W2; 36.
DR STRING; 6239.Y62F5A.1a; -.
DR EPD; Q9U1W2; -.
DR PaxDb; Q9U1W2; -.
DR PeptideAtlas; Q9U1W2; -.
DR EnsemblMetazoa; Y62F5A.1a.1; Y62F5A.1a.1; WBGene00007013. [Q9U1W2-2]
DR EnsemblMetazoa; Y62F5A.1b.1; Y62F5A.1b.1; WBGene00007013. [Q9U1W2-1]
DR GeneID; 174763; -.
DR KEGG; cel:CELE_Y62F5A.1; -.
DR UCSC; Y62F5A.1a; c. elegans. [Q9U1W2-1]
DR CTD; 174763; -.
DR WormBase; Y62F5A.1a; CE24544; WBGene00007013; mdt-8. [Q9U1W2-2]
DR WormBase; Y62F5A.1b; CE24545; WBGene00007013; mdt-8. [Q9U1W2-1]
DR eggNOG; KOG3583; Eukaryota.
DR GeneTree; ENSGT00390000011838; -.
DR HOGENOM; CLU_799832_0_0_1; -.
DR InParanoid; Q9U1W2; -.
DR OMA; QEEVEWP; -.
DR OrthoDB; 1392867at2759; -.
DR PhylomeDB; Q9U1W2; -.
DR SignaLink; Q9U1W2; -.
DR PRO; PR:Q9U1W2; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00007013; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR019364; Mediatior_Med8_fun/met.
DR PANTHER; PTHR13074; PTHR13074; 1.
DR Pfam; PF10232; Med8; 1.
PE 3: Inferred from homology;
KW Activator; Alternative splicing; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..297
FT /note="Mediator of RNA polymerase II transcription subunit
FT 8"
FT /id="PRO_0000304532"
FT REGION 204..297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..297
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 294..297
FT /note="KFQQ -> TMMAPGQMIGHPIQQQRPPMHQMPPNMTIQRQ (in isoform
FT a)"
FT /evidence="ECO:0000305"
FT /id="VSP_028028"
SQ SEQUENCE 297 AA; 33438 MW; 1F7DE3015B6EA8ED CRC64;
MEYPNPPPPV TSNYQGEPEK IAQATDMMIK RVTDAKKIIE ELLQMLDLQE KCPWPLMLEK
FSTLASFMSS LQSSVRKSGM PHGHEDYGQF LRSHVLVTQR LQYEPDETLQ RATQGRVMSW
NHALVPEYLR TKPNPEMENE EGMLDGERSA KSADLVVRQI AAYNKNIEGL INHLNSVDRM
HTEAAIEKPT YNRDETAKIV KSILTGEGIR SQRTMAPAPP SSAPMTTAPS STGPSSSQPI
SGNQPEYQGS QLRQQLSGGQ PQTSQAHLPM YSHHQQPQYS HQQPMNPQHH SPMKFQQ
