ID   MED8_CANAL              Reviewed;         239 AA.
AC   Q59TD3; A0A1D8PH66; Q59TG7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE   AltName: Full=Mediator complex subunit 8;
GN   Name=MED8; OrderedLocusNames=CAALFM_C204530WA;
GN   ORFNames=CaO19.11973, CaO19.4497;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000305}.
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DR   EMBL; CP017624; AOW27479.1; -; Genomic_DNA.
DR   RefSeq; XP_712923.2; XM_707830.2.
DR   AlphaFoldDB; Q59TD3; -.
DR   SMR; Q59TD3; -.
DR   BioGRID; 1228539; 27.
DR   STRING; 237561.Q59TD3; -.
DR   PRIDE; Q59TD3; -.
DR   GeneID; 3645446; -.
DR   KEGG; cal:CAALFM_C204530WA; -.
DR   CGD; CAL0000185831; MED8.
DR   VEuPathDB; FungiDB:C2_04530W_A; -.
DR   eggNOG; ENOG502S8U1; Eukaryota.
DR   HOGENOM; CLU_108151_0_0_1; -.
DR   InParanoid; Q59TD3; -.
DR   OMA; PQWYSLQ; -.
DR   OrthoDB; 1472120at2759; -.
DR   PRO; PR:Q59TD3; -.
DR   Proteomes; UP000000559; Chromosome 2.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; PTHR13074; 1.
DR   Pfam; PF10232; Med8; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..239
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   8"
FT                   /id="PRO_0000304538"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          30..59
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   239 AA;  27959 MW;  EFDF7C7331CBB019 CRC64;
     MSQTPFNPNS PSVPTTTSLP STDFSQIPYD VLESIRNRLN QIRQSLTKLA DQINNHNRHP
     QKIKLPSYSY FQNQFQVLIT QLMSITNQLY NNEDLLRNTN VYPTPIFPTS AHENMLTTLL
     RKKVAPEVDE WINGAKLIME NETKNKLDNQ QVEIDILKID QWVQWCLAKI QDLRDEFQFY
     GFHTVEELDH METPQGKQEI KEKKDKEFER EQIELKITAG GQKSLHPNQV LKFMCQGQI