ID   MED8_CHAGB              Reviewed;         288 AA.
AC   Q2HDD6;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE   AltName: Full=Mediator complex subunit 8;
GN   Name=MED8; ORFNames=CHGG_01768;
OS   Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 /
OS   NRRL 1970) (Soil fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Chaetomiaceae; Chaetomium.
OX   NCBI_TaxID=306901;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970;
RX   PubMed=25720678; DOI=10.1128/genomea.00021-15;
RA   Cuomo C.A., Untereiner W.A., Ma L.-J., Grabherr M., Birren B.W.;
RT   "Draft genome sequence of the cellulolytic fungus Chaetomium globosum.";
RL   Genome Announc. 3:E0002115-E0002115(2015).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000305}.
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DR   EMBL; CH408029; EAQ93533.1; -; Genomic_DNA.
DR   RefSeq; XP_001220989.1; XM_001220988.1.
DR   AlphaFoldDB; Q2HDD6; -.
DR   SMR; Q2HDD6; -.
DR   STRING; 38033.XP_001220989.1; -.
DR   EnsemblFungi; EAQ93533; EAQ93533; CHGG_01768.
DR   GeneID; 4386751; -.
DR   eggNOG; ENOG502S8U1; Eukaryota.
DR   HOGENOM; CLU_074399_0_0_1; -.
DR   InParanoid; Q2HDD6; -.
DR   OMA; WAPIEAN; -.
DR   OrthoDB; 1472120at2759; -.
DR   Proteomes; UP000001056; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; PTHR13074; 1.
DR   Pfam; PF10232; Med8; 1.
PE   3: Inferred from homology;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..288
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   8"
FT                   /id="PRO_0000304540"
FT   REGION          122..150
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          191..245
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          266..288
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          4..58
FT                   /evidence="ECO:0000255"
FT   COILED          180..228
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        135..149
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        200..222
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   288 AA;  31560 MW;  CCA9B516155B8E79 CRC64;
     MSPEELKQLE LLRNRFAQLT SNLGSLRTSV SNSNPLPTYE SLQASVNILQ ANIRSIQDIA
     TENAGLFQRL AIHPSTNFPG RTQEHILTQL LRKKLEPNVE SWVEEARATA RGVGVDASKL
     AGGVHRRGEH DYDDEGTYGM DEDGDDAPQD PFSEQWADML DNFQQTLQQY VTVQVKKKYT
     AEERAAGIEN VRTGLRQTLE ESEDDDEDEE EEEEDEEEDE AAAAAGNAGG LATGAGGSAA
     AGGGMFGAIE PEHIFYLQAQ GNLQLPGNVP IESKRIQTVP TRRVAPPR