ID   MED8_DANRE              Reviewed;         267 AA.
AC   Q6NYT1;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE   AltName: Full=Mediator complex subunit 8;
GN   Name=med8; ORFNames=zgc:76877;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors. May play a role as a target
CC       recruitment subunit in E3 ubiquitin-protein ligase complexes and thus
CC       in ubiquitination and subsequent proteasomal degradation of target
CC       proteins (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Component of the Mediator complex. May be part of a
CC       multisubunit E3 ubiquitin-protein ligase complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000305}.
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DR   EMBL; BC066472; AAH66472.1; -; mRNA.
DR   RefSeq; NP_996966.1; NM_207083.1.
DR   AlphaFoldDB; Q6NYT1; -.
DR   SMR; Q6NYT1; -.
DR   STRING; 7955.ENSDARP00000027433; -.
DR   PaxDb; Q6NYT1; -.
DR   GeneID; 404615; -.
DR   KEGG; dre:404615; -.
DR   CTD; 112950; -.
DR   ZFIN; ZDB-GENE-040426-2091; med8.
DR   eggNOG; KOG3583; Eukaryota.
DR   InParanoid; Q6NYT1; -.
DR   OrthoDB; 1392867at2759; -.
DR   PhylomeDB; Q6NYT1; -.
DR   Reactome; R-DRE-212436; Generic Transcription Pathway.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q6NYT1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; PTHR13074; 1.
DR   Pfam; PF10232; Med8; 1.
PE   2: Evidence at transcript level;
KW   Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Ubl conjugation pathway.
FT   CHAIN           1..267
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   8"
FT                   /id="PRO_0000304525"
FT   REGION          156..180
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          227..267
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          2..27
FT                   /evidence="ECO:0000255"
FT   COILED          118..163
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        245..267
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   267 AA;  29402 MW;  330278074132AFAF CRC64;
     MQQREEKQLE AFLESLVARV AHLKGSLQSF IYKLENEYDR LTWPSVLDNF ALISGQLNTI
     NKLLRNEKTP SYKSQVIIPL LLSPDRDEEL AKLTEHRVPV FSHEIVPDHL RTKPDPEVEE
     QEKHLSAEAA RIGPEVAQKQ IQALNKLCSN LLEKLNNPRE DRDSETSALR QNKPSFNPAD
     TNALVAAVGF GKGLSKCRPP GPVAPGHPGQ PMMQTGPTLQ QVTIAGASGH QAGMSGPVAP
     QQPGQPGKIP SNIKTNIKSA SMHPYNR