MED8_DEBHA
ID MED8_DEBHA Reviewed; 225 AA.
AC Q6BM45; B5RUC3;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 2.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE AltName: Full=Mediator complex subunit 8;
GN Name=MED8; OrderedLocusNames=DEHA2F08404g;
OS Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990
OS / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX NCBI_TaxID=284592;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC {ECO:0000305}.
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DR EMBL; CR382138; CAR66301.1; -; Genomic_DNA.
DR RefSeq; XP_002770775.1; XM_002770729.1.
DR AlphaFoldDB; Q6BM45; -.
DR SMR; Q6BM45; -.
DR STRING; 4959.XP_002770775.1; -.
DR PRIDE; Q6BM45; -.
DR EnsemblFungi; CAR66301; CAR66301; DEHA2F08404g.
DR GeneID; 8998918; -.
DR KEGG; dha:DEHA2F08404g; -.
DR VEuPathDB; FungiDB:DEHA2F08404g; -.
DR eggNOG; ENOG502S8U1; Eukaryota.
DR HOGENOM; CLU_108151_0_0_1; -.
DR InParanoid; Q6BM45; -.
DR OMA; PQWYSLQ; -.
DR OrthoDB; 1472120at2759; -.
DR Proteomes; UP000000599; Chromosome F.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR InterPro; IPR019364; Mediatior_Med8_fun/met.
DR PANTHER; PTHR13074; PTHR13074; 1.
DR Pfam; PF10232; Med8; 1.
PE 3: Inferred from homology;
KW Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..225
FT /note="Mediator of RNA polymerase II transcription subunit
FT 8"
FT /id="PRO_0000304542"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 170..204
FT /evidence="ECO:0000255"
SQ SEQUENCE 225 AA; 26203 MW; 64F6638767450DF5 CRC64;
MNSIHSANSN TVSTSEINTS QIPTDSLESI RNRLNQVHLS LRKLSDQINH HNRHPNKVKL
PNYAQFQNQF QVLLTQLHTI TSDLENNDEL LKNTNVYPLP SFPTTQQEGL ITTLLRKKPL
PEVDEWIESA IDKRKNEYVN VQKDDEFAQW CLTKIEELRE DFQFYGFNSV EELKYLDSED
EKKETEENKK IENQKYEEEL KITAGGKSGL HPNRVLKFMC QGVLT