MED8_DICDI
ID MED8_DICDI Reviewed; 524 AA.
AC Q54B97;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Putative mediator of RNA polymerase II transcription subunit 8;
DE AltName: Full=Putative mediator complex subunit 8;
GN Name=med8; ORFNames=DDB_G0293824;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NOMENCLATURE.
RX PubMed=18515835; DOI=10.1093/nar/gkn349;
RA Bourbon H.-M.;
RT "Comparative genomics supports a deep evolutionary origin for the large,
RT four-module transcriptional mediator complex.";
RL Nucleic Acids Res. 36:3993-4008(2008).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. May play a role as a target
CC recruitment subunit in E3 ubiquitin-protein ligase complexes and thus
CC in ubiquitination and subsequent proteasomal degradation of target
CC proteins (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Component of the Mediator complex. May be part of a
CC multisubunit E3 ubiquitin-protein ligase complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000220; EAL60545.1; -; Genomic_DNA.
DR RefSeq; XP_628953.1; XM_628951.1.
DR AlphaFoldDB; Q54B97; -.
DR SMR; Q54B97; -.
DR PaxDb; Q54B97; -.
DR EnsemblProtists; EAL60545; EAL60545; DDB_G0293824.
DR GeneID; 8629430; -.
DR KEGG; ddi:DDB_G0293824; -.
DR dictyBase; DDB_G0293824; med8.
DR HOGENOM; CLU_520179_0_0_1; -.
DR InParanoid; Q54B97; -.
DR OMA; SKNSTHY; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q54B97; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
PE 3: Inferred from homology;
KW Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Ubl conjugation pathway.
FT CHAIN 1..524
FT /note="Putative mediator of RNA polymerase II transcription
FT subunit 8"
FT /id="PRO_0000388648"
FT REGION 312..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 356..379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 430..451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 117..146
FT /evidence="ECO:0000255"
FT COILED 175..211
FT /evidence="ECO:0000255"
FT COILED 398..478
FT /evidence="ECO:0000255"
FT COMPBIAS 356..377
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 524 AA; 58691 MW; B9FE1C4917FF1ED1 CRC64;
MNGVDINSNL DSIKAKAEDI RNLLLTTSTG NWEDILGHFV VLSGEYGSLV REVNNIYDSV
AVFPEQVTGD PNLIPNLLRT KLSPELETKN RDIINQYVSK NSTHYDPGNQ EENIPPLKLH
IKSFNDRIDK VDQEFENKIQ QISKHKLTKP SASHDTAILK DILSITMLGS GIKINAQQQL
AQQQQQQATI NALQQQQRLL NAQQQQQQQQ QPQALYKLPA TNTANTSTNT PSSTIVINSP
SPGGIHHNVP TPPTNLNAAG TRTSPPNVQQ YVNSPPPQQQ QQQQINTVIG TPNTIPTTPT
ATVSTAATYK PVASPQQQVT SKQVPIQSTN KPLPQQQPSI QIQPQQSIVQ MVQNVLPNTT
SPPVNNNNQS PINSGIGGGQ FKPSAINIAN ANSGTSTIQQ QIQLQQQQLQ QQIQLQQQLA
QQQQQNQQNQ QNQQNQQNQQ NQQIHQHIQH LTPQQQAQLQ QQMLQQQQLQ QQFQQQQLSQ
QLLQQQQLHQ QQQPNQTIQN PFHNFQANNF RVQNPTNFNP PQNQ
