MED8_DROME
ID MED8_DROME Reviewed; 252 AA.
AC A1ZBT5; Q8SZ54;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 8;
DE AltName: Full=Mediator complex subunit 8;
GN Name=MED8; Synonyms=Arc32; ORFNames=CG13867;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION.
RX PubMed=16751183; DOI=10.1101/gad.1418806;
RA Marr M.T. II, Isogai Y., Wright K.J., Tjian R.;
RT "Coactivator cross-talk specifies transcriptional output.";
RL Genes Dev. 20:1458-1469(2006).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors (By similarity). Required for
CC activated transcription of the MtnA and MtnB genes. {ECO:0000250,
CC ECO:0000269|PubMed:16751183}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC -!- INTERACTION:
CC A1ZBT5; Q9VP65: CG12975; NbExp=3; IntAct=EBI-90766, EBI-141782;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC {ECO:0000305}.
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DR EMBL; AE013599; AAM68406.1; -; Genomic_DNA.
DR EMBL; AY071108; AAL48730.1; -; mRNA.
DR RefSeq; NP_722456.1; NM_153772.2.
DR AlphaFoldDB; A1ZBT5; -.
DR SMR; A1ZBT5; -.
DR BioGRID; 62960; 41.
DR IntAct; A1ZBT5; 9.
DR STRING; 7227.FBpp0085609; -.
DR PaxDb; A1ZBT5; -.
DR PRIDE; A1ZBT5; -.
DR DNASU; 37305; -.
DR EnsemblMetazoa; FBtr0086297; FBpp0085609; FBgn0034503.
DR GeneID; 37305; -.
DR KEGG; dme:Dmel_CG13867; -.
DR CTD; 112950; -.
DR FlyBase; FBgn0034503; MED8.
DR VEuPathDB; VectorBase:FBgn0034503; -.
DR eggNOG; KOG3583; Eukaryota.
DR HOGENOM; CLU_085476_0_0_1; -.
DR InParanoid; A1ZBT5; -.
DR OMA; IIMRVND; -.
DR OrthoDB; 1392867at2759; -.
DR PhylomeDB; A1ZBT5; -.
DR BioGRID-ORCS; 37305; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 37305; -.
DR PRO; PR:A1ZBT5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034503; Expressed in eye disc (Drosophila) and 25 other tissues.
DR ExpressionAtlas; A1ZBT5; baseline and differential.
DR Genevisible; A1ZBT5; DM.
DR GO; GO:0070847; C:core mediator complex; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; ISS:FlyBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR InterPro; IPR019364; Mediatior_Med8_fun/met.
DR PANTHER; PTHR13074; PTHR13074; 1.
DR Pfam; PF10232; Med8; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..252
FT /note="Mediator of RNA polymerase II transcription subunit
FT 8"
FT /id="PRO_0000304531"
FT REGION 215..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 117..142
FT /evidence="ECO:0000255"
FT COMPBIAS 221..252
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 151
FT /note="L -> P (in Ref. 3; AAL48730)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 252 AA; 27948 MW; 876320D1BA3418F4 CRC64;
MQRDEKLFEL TLDTVLQRLN DLKLAVLSMI QKLELEYETI NWPTFLDNFA IISSHLTGLT
KILAKEQCPP LRNRTVLPLL VSMDRDDTLI NITEGRVPVF SHDIVPDYLR TRPDPITEQK
MLQNEQKAAN LTNDAAMKQV TQYNKVVSHV LDMVSKAREE WEIESSSRTG IQQTSSMADT
QLLVAAVGMG KGLKLTNYGP GPGMMVPPSI RAPSPMGGPA MSPGNVQQQL GKAPSAVKTN
IKSANQVHPF SR
